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货号： bs-1309R 基本售价： 380.0 元 规格： 20ul
- 规格：20ul
- 价格：380.00元
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1309R
- 英文名称
- Syndecan-1
- 中文名称
- 多配体蛋白聚糖1抗体
- 别 名
- SDC1; SDC; SYND1; Syndecan I; SyndecanI; Syndecan-I; syndecan; Sdc1; Syndecan1; AA408134; AA409076; Sstn; syn-1; CD138; CD 138; CD-138; CD138 antigen; Heparan sulfate proteoglycan fibroblast growth factor receptor; SDC 1; Syndecan1 precursor; SDC1_HUMAN.
- Specific References (3) | bs-1309R has been referenced in 3 publications.[IF=2.65] Miyatani, Kozo, et al. "A high number of IgG4-positive cells in gastric cancer tissue is associated with tumor progression and poor prognosis." Virchows Archiv (2016): 1-9. IHC-F ; Human.PubMed:26951261[IF=2.54] Chen, Song, et al. "Heparanase Mediates Intestinal Inflammation and Injury in a Mouse Model of Sepsis." Journal of Histochemistry & Cytochemistry (2017): 0022155417692536. IHC-P ; Mouse.PubMed:28170292[IF=2.97] Hambruch, N., et al. "Bovine placentomal heparanase and syndecan expression is related to placental maturation." Placenta. IHC-P ; Bovine.PubMed:0
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 神经生物学 细胞膜受体 细胞粘附分子 细胞表面分子 糖蛋白 上皮细胞 内皮细胞
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 34kDa
- 细胞定位
- 细胞膜 分泌型蛋白
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human CD138:201-310/310 <Cytoplasmic>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008].
Function:
Cell surface proteoglycan that bears both heparin sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.
Subunit:
Interacts with CDCP1.
Subcellular Location:
Membrane; Single-pass type I membrane protein. Secreted. Note=Shedding of the ectodomain produces a soluble form.
Post-translational modifications:
Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.
Similarity:
Belongs to the syndecan proteoglycan family.
SWISS:
P18827
Gene ID:
6382
Database links:Entrez Gene: 6382 Human
Entrez Gene: 20969 Mouse
Entrez Gene: 25216 Rat
Omim: 186355 Human
SwissProt: P18827 Human
SwissProt: P18828 Mouse
SwissProt: P26260 Rat
Unigene: 224607 Human
Unigene: 665958 Human
Unigene: 2580 Mouse
Unigene: 11176 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Syndecan1(CD138)属于Syndecans基因家族，该家族是一类细胞表面跨膜蛋白多糖 ,是膜表面粘附受体 ,参与细胞-细胞外基质之间的粘附。
CD138表达于上皮细胞、胚胎间质细胞、血管平滑肌细胞、内皮细胞和神经细胞基底膜。Syndecan-1与恶性肿瘤的发生发展、浸润及转移有关，着色于胞浆和胞膜.
- 产品图片
Sample:
JEG-3(Human) Cell Lysate at 30 ug
Primary: Anti-Syndecan-1 (bs-1309R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Syndecan-1) Polyclonal Antibody, Unconjugated (bs-1309R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Syndecan-1) Polyclonal Antibody, Unconjugated (bs-1309R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Syndecan-1) Polyclonal Antibody, Unconjugated (bs-1309R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).