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货号： bs-6293R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-6293R
- 英文名称
- Prostatic Acid Phosphatase
- 中文名称
- 前列腺酸性磷酸酶抗体
- 别 名
- Acid phosphatase prostate; ACP 3; ACP3; ACPP; Ecto-5-nucleotidase; PAP; PPAP_HUMAN; Prostatic acid phosphatase; Thiamine monophosphatase; TMPase; 5-NT; 5-nucleotidase.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 激酶和磷酸酶 肿瘤细胞生物标志物
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Horse,
- 产品应用
- WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 41kDa
- 细胞定位
- 细胞膜 分泌型蛋白
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Prostatic Acid Phosphatase:21-120/386
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Prostatic Acid Phosphatase is Belongs to the histidine acid phosphatase family.Cellular localization;Secreted and Lysosome membrane. Predominantly localized in the plasma membrane but also detected in intracellular vesicles.
Function:
A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.
Subunit:
Homodimer; dimer formation is required for phosphatase activity.
Subcellular Location:
Isoform 1: Secreted. Isoform 2: Cell membrane.
Isoform 2: the cellular form also has ecto-5-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor. Acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling.
Tissue Specificity:
Highly expressed in the prostate, restricted to glandular and ductal epithelial cells.
Post-translational modifications:
N-glycosylated. High mannose content, partially sialylated and fucosylated biantennary complex. Also fucosylated with partially sialylated triantennary complex oligosaccharides.
Proteolytically cleaved in seminal fluid to produce several peptides. Peptide PAPf39, the most prominent, forms amyloid beta-sheet fibrils, SEVI (semen-derived enhancer of viral infection) which entrap HIV virions, attach them to target cells and enhance infection. SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea. Target cell attachment and enhancement of HIV infection is inhibited by surfen. Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection.
Similarity:
Belongs to the histidine acid phosphatase family.
SWISS:
P24666
Gene ID:
55
Database links:Entrez Gene: 504700 Cow
Entrez Gene: 55 Human
Entrez Gene: 56318 Mouse
Entrez Gene: 56780 Rat
Omim: 171790 Human
SwissProt: A6H730 Cow
SwissProt: P15309 Human
SwissProt: Q8CE08 Mouse
SwissProt: P20646 Rat
Unigene: 433060 Human
Unigene: 19941 Mouse
Unigene: 40121 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample: PC3 Cell (Human) Lysate at 40 ug
Primary: Anti-Prostatic Acid Phosphatase (bs-6293R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (Human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Prostatic Acid Phosphatase) Polyclonal Antibody, Unconjugated (bs-6293R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.