产品中心
当前位置：首页>产品中心Anti-ZNF185
货号： bs-6119R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-6119R
- 英文名称
- ZNF185
- 中文名称
- 锌指蛋白185抗体
- 别 名
- LIM domain protein ZNF185; P1 A; Zinc finger protein 185 (LIM domain); ZN185_HUMAN.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 染色质和核信号 信号转导
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3ug/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 76(human)/39(mo,kDa
- 细胞定位
- 细胞浆 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human ZNF185:551-650/689
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
ZNF185 encodes a LIM domain zinc finger protein. It may be involved in the regulation of cellular proliferation and/or differentiation.
Function:
May be involved in the regulation of cellular proliferation and/or differentiation.
Subcellular Location:
Cytoplasm, cytoskeleton. Cell junction, focal adhesion.
Tissue Specificity:
Expressed in placenta, pancreas and kidney. Also expressed in prostate, testis, ovary and blood.
Similarity:
Contains 1 LIM zinc-binding domain.
SWISS:
O15231
Gene ID:
7739
Database links:Entrez Gene: 7739 Human
Entrez Gene: 22673 Mouse
Omim: 300381 Human
SwissProt: O15231 Human
SwissProt: Q62394 Mouse
Unigene: 16622 Human
Unigene: 1161 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
MCF-7(Human) Cell Lysate at 40 ug
A431(Human) Cell Lysate at 40 ug
U2os(Human) Cell Lysate at 40 ug
Primary: Anti-ZNF185 (bs-6119R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 76/39 kD
Observed band size: 67 kD
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-Phospho-c-Fos (Ser32) antibody (bs-6199R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.