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货号： bs-4265R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-4265R
- 英文名称
- E2F8
- 中文名称
- 转录因子E2F8抗体
- 别 名
- E2F 8; E2F family member 82; E2F transcription factor 8; E2F-8; E2f8; E2F8_HUMAN; FLJ23311; Transcription factor E2F8.
- Specific References (1) | bs-4265R has been referenced in 1 publications.[IF=4.20] Yuan, Qing, et al. "Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity." International Journal of Nanomedicine 9 (2014): 4829. WB ; Mouse.PubMed:25378924
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 免疫学 染色质和核信号 信号转导 转录调节因子 新陈代谢 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Pig, Horse, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 94kDa
- 细胞定位
- 细胞核
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human E2F8:265-360/968
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene encodes a member of a family of transcription factors which regulate the expression of genes required for progression through the cell cycle. The encoded protein regulates progression from G1 to S phase by ensuring the nucleus divides at the proper time. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2012].
Function:
Along with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP proteins through the E2 recognition site, 5-TTTC[CG]CGC-3. Directly represses E2F1 transcription. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession.
Subunit:
Forms heterodimers with E2F7 and, to a lesser extent, homodimers. Dimerization is important for DNA binding.
Subcellular Location:
Nucleus.
Similarity:
Belongs to the E2F/DP family
SWISS:
A0AVK6
Gene ID:
79733
Database links:Entrez Gene: 79733 Human
Entrez Gene: 108961 Mouse
Entrez Gene: 308607 Rat
Omim: 612047 Human
SwissProt: A0AVK6 Human
SwissProt: Q58FA4 Mouse
Unigene: 523526 Human
Unigene: 240566 Mouse
Unigene: 395068 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
Kidney (Rat) Lysate at 40 ug
Brain (Rat) Lysate at 40 ug
Primary: Anti-E2F8 (bs-4265R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 94 kD
Observed band size: 93 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (E2F8) Polyclonal Antibody, Unconjugated (bs-4265R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.