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货号： bs-5493R 基本售价： 1580.0 元 规格： 100ul
产品信息
- 产品编号
- bs-5493R
- 英文名称
- phospho-MAP4K4 (Ser801)
- 中文名称
- 磷酸化丝裂原活化蛋白激酶MAP4K4抗体
- 别 名
- MAP4K4(phospho Ser801); MAP4K4(phospho S801); Hepatocyte progenitor kinase like/germinal center kinase like kinase; HGK; HPK/GCK like kinase; HPK/GCK like kinase HGK; KIAA0687; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; MEKKK4; Mitogen activated protein kinase kinase kinase kinase 4; Nck interacting kinase; NIK.
- 规格价格
- 100ul/1580元购买 大包装/询价
- 说 明 书
- 100ul
- 产品类型
- 磷酸化抗体
- 研究领域
- 肿瘤 免疫学 信号转导 转录调节因子 激酶和磷酸酶
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Horse, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 142kDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated Synthesised phosphopeptide derived from human MAP4K4 around the phosphorylation site of Ser801:AS(p-S)LN
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
MAP4K4 (Mitogen-activated protein kinase kinase kinase kinase 4) belongs to the serine/threonine protein kinase family and specifically activates MAPK8/JNK. It may play a role in the response to environmental stress and is thought to function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF alpha signaling pathway. It interacts with the SH3 domain of the adapter protein Nck. MAP4K4-dependent signaling inhibits PPARG responsive gene expression, adipogenesis, and insulin stimulated glucose transport.
Function:
Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322.
Subunit:
Interacts with the SH3 domain of the adapter proteins Nck (By similarity). Interacts (via its CNH regulatory domain) with ATL1 (via the N-terminal region). Interacts with RAP2A (GTP-bound form preferentially).
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity:
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain.
Contains 1 protein kinase domain.
SWISS:
O95819
Gene ID:
9448
Database links:Entrez Gene: 9448 Human
Omim: 604666 Human
SwissProt: O95819 Human
Unigene: 431550 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
Hela Cell (Human) Lysate at 30 ug
HepG2 Cell (Human) Lysate at 30 ug
Primary: Anti- p-MAP4K4 (Ser801) (bs-5493R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 142kD
Observed band size: 135kD
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phosphor-MAP4K4) Polyclonal Antibody, Unconjugated (bs-5493R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.