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货号： bs-8479R-PE-Cy5.5 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-8479R-PE-Cy5.5
- 英文名称
- Anti-GTF2F1/PE-Cy5.5
- 中文名称
- PE-Cy5.5标记的转录起始因子RAP30抗体
- 别 名
- TFIIF RAP30; ATP dependent helicase GTF2F2; BTF 4; BTF4; General transcription factor IIF polypeptide 1; General transcription factor IIF polypeptide 1 74 kDa subunit protein; General transcription factor IIF polypeptide 1 74kDa; General transcription factor IIF polypeptide 2 30kDa; General Transcription Factor IIF Polypeptide 2; GTF2F1; GTF2F1 protein; GTF2F2; RAP 30; RAP 74; RAP30; RAP74; TF2F 1; TF2F 2; TF2F1; TF2F2; TFIIF alpha; TFIIF beta; Transcription initiation factor IIF alpha subunit; Transcription Initiation Factor IIF beta Subunit; Transcription initiation factor IIF subunit beta; Transcription initiation factor RAP30; Transcription initiation factor RAP74; T2FB_HUMAN.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 研究领域
- 细胞生物 转录调节因子 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Cow, Horse, Rabbit,
- 产品应用
- ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 28kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human TFIIF RAP30
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. TFIIF, a heteromer composed of a small (RAP 30) and a large (RAP 74) subunit, is required for RNA polymerase II to assemble into a preinitiation complex formed by promotor DNA and the general factors TFIID, IIA and IIB. In addition, TFIIF stimulates transcription elongation by RNA polymerase II.
Function:
The RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD. TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template. TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.
Subunit:
Heterodimer of an alpha and a beta subunit. Interacts with HTATSF1 and GPBP1 (By similarity). Interacts with URI1.
Subcellular Location:
Nuclear
Similarity:
Belongs to the TFIIF beta subunit family.
Database links:Entrez Gene: 2962Human
Entrez Gene: 505702Cow
Entrez Gene: 2963Human
Entrez Gene: 68705Mouse
Entrez Gene: 98053Mouse
Entrez Gene: 316123Rat
Entrez Gene: 81674Rat
Omim: 189968Human
Omim: 189969Human
SwissProt: Q5EA53Cow
SwissProt: P13984Human
SwissProt: P35269Human
SwissProt: Q8BVJ2Mouse
SwissProt: Q8R5B7Mouse
SwissProt: Q01750Rat
SwissProt: Q6AY96Rat
Unigene: 654582Human
Unigene: 68257Human
Unigene: 24632Mouse
Unigene: 98836Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.