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货号： bs-5084R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-5084R
- 英文名称
- ME1
- 中文名称
- 胞浆苹果酸酶1抗体
- 别 名
- Cytosolic malic enzyme 1; HUMNDME; Malate dehydrogenase; Malic enzyme 1; Malic enzyme 1, NADP(+) dependent, cytosolic; Malic enzyme 1, soluble; Malic enzyme, cytoplasmic; MES; NADP dependent malic enzyme; NADP ME; Pyruvic malic carboxylase.Cytosolic malic enzyme 1; HUMNDME; Malate dehydrogenase; Malic enzyme 1; Malic enzyme 1, NADP(+) dependent, cytosolic; Malic enzyme 1, soluble; Malic enzyme, cytoplasmic; MES; NADP dependent malic enzyme; NADP ME; Pyruvic malic carboxylase.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 免疫学 染色质和核信号 转录调节因子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 64kDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human NADP ME:451-550/572
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
ME1 is a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Expressed in all tissues tested including liver, placenta and white adipose tissue.
Similarity:
Belongs to the malic enzymes family.
SWISS:
P48163
Gene ID:
4199
Database links:Entrez Gene: 4199Human
Entrez Gene: 17436Mouse
Entrez Gene: 24552Rat
Omim: 154250Human
SwissProt: P48163Human
SwissProt: P06801Mouse
SwissProt: P13697Rat
Unigene: 21160Human
Unigene: 148155Mouse
Unigene: 3519Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
Hela(human)cell Lysate at 30 ug
Primary: Anti- ME1 (bs-5084R)at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64kD
Observed band size: 64kD
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ME1) Polyclonal Antibody, Unconjugated (bs-5084R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.