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货号： bs-3666R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-3666R
- 英文名称
- Calmodulin
- 中文名称
- 钙调节素/钙调蛋白/钙调素抗体
- 别 名
- CALM 1; CALM 2; CALM 3; CALM; CALM1; CALM2; CALM3; CALML2; Calmodulin 1; Calmodulin1; CAM 1; CAM 2; CAM 3; CaM; CAM I; CAM1; CAM2; CAM3; CAMB; CAMC; CAMI; CAMIII; DD132; PHKD; Phosphorylase kinase delta; Phosphorylase kinase delta subunit; Calmodulin1; Calmodulin 1; Calmodulin-1; Calmodulin2; Calmodulin 2; Calmodulin-2; Calmodulin3; Calmodulin 3; Calmodulin-3; CALM1_HUMAN.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 免疫学 生长因子和激素 转录调节因子 细胞表面分子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Cow, Horse,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 16-20kDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Calmodulin:81-152/152
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Calmodulin consists of two glycoproteins, 34 and 39 kDa, sometimes designated epithelial antigen, epithelial specific antigen, and epithelial glycoprotein. The glycoproteins are located on the cell membrane surface and in the cytoplasm of virtually all epithelial cells with the exception of most squamous epithelia, hepatocytes, renal proximal tubular cells, gastric parietal cells and myoepithelial cells. Epithelial Calmodulin is found in the large majority of adenocarcinomas of most sites (50-100% in various studies; as well as neuroendocrine tumours, including small cell carcinoma. Renal cell carcinoma and hepatocellular carcinoma stain in about 30% of the cases. Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin Ca(2+) complex are a number of protein kinases and phosphatases. Calmodulin has four functional calcium binding sites.
Subunit:
Interacts with MYO1C and RRAD. Interacts with MYO10 (By similarity). Interacts with CEP97, CEP110, TTN/titin and SRY. Interacts with USP6; the interaction is calcium dependent. Interacts with CDK5RAP2. Interacts with SCN5A. Interacts with RYR1 and RYR2.
Subcellular Location:
Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Note=Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
Similarity:
Belongs to the calmodulin family. Contains 4 EF-hand domains.
SWISS:
P62158
Gene ID:
801
Database links:Entrez Gene: 801 Human
Entrez Gene: 12314 Mouse
Entrez Gene: 12315 Mouse
Entrez Gene: 24242 Rat
Entrez Gene: 24244 Rat
Entrez Gene: 50663 Rat
Omim: 114180 Human
Omim: 114182 Human
Omim: 114183 Human
SwissProt: P62158 Human
SwissProt: P62204 Mouse
SwissProt: P62161 Rat
Unigene: 282410 Human
Unigene: 468442 Human
Unigene: 515487 Human
Unigene: 706125 Human
Unigene: 722012 Human
Unigene: 285993 Mouse
Unigene: 288630 Mouse
Unigene: 329243 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
钙离子调节素（calmodulin,CaM）: 钙调蛋白（CaM），是由一条多肽链组成的单体蛋白。可结合4个Ca2+，胞浆Ca2+浓度≥10-2mmol/L时，Ca2+与CaM结合，构象改变而激活Ca2+ -CaM激酶。
- 产品图片
Sample:
Lane1: Brain(Rat) Lysate at 30 ug
Lane2: Liver(Rat) Lysate at 30 ug
Primary: Anti-Calmodulin (bs-3666R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 16kD
Observed band size : 20kD
Tissue/cell:Human hepatocellular carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Calmodulin Polyclonal Antibody, Unconjugated(bs-3666R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining