Anti-Phospho-TORC2 (Ser171)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Anti-Phospho-TORC2 (Ser171)

货号： bs-3415R 基本售价： 1580.0 元规格： 100ul

产品信息

产品编号: bs-3415R

英文名称: Phospho-TORC2 (Ser171)

中文名称: 磷酸化CREB转录共激活因子TORC2抗体

别名: TORC2 (phospho S171); TORC2 (phospho Ser171); p-TORC2 (Ser171); CREB regulated transcription coactivator 2; CRTC2; RP11-422P24.6; TORC-2; Transducer of CREB protein 2; Transducer of regulated cAMP response element-binding protein; Transducer of regulated cAMP response element-binding protein (CREB) 2; Transducer of regulated cAMP response element-binding protein 2; Transducer of regulated CREB protein 2; CRTC2_HUMAN.

: Specific References  (2)     |     bs-3415R has been referenced in 2 publications.
[IF=2.09] Tian, Xiaohui, et al. "CRTC2 enhances HBV transcription and replication by inducing PGC1alpha expression." Virology Journal 11.1 (2014): 30.  WB ;  Human.
PubMed:24529027
[IF=3.02] Slocum, Stephen L., et al. "Keap1/Nrf2 pathway activation leads to a repressed hepatic gluconeogenic and lipogenic program in mice on a high-fat diet." Archives of Biochemistry and Biophysics (2016).  WB ;  Mouse.
PubMed:26701603

规格价格: 100ul/1580元购买大包装/询价

说明书: 100ul

产品类型: 磷酸化抗体

研究领域: 肿瘤免疫学信号转导细胞凋亡转录调节因子激酶和磷酸酶

抗体来源: Rabbit

克隆类型: Polyclonal

交叉反应: Human, Mouse, Rat, Dog, Pig,

产品应用: ELISA=1:500-1000 Flow-Cyt=1ug/Test （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分子量: 73kDa

细胞定位: 细胞核细胞浆

性状: Lyophilized or Liquid

浓度: 1mg/ml

免疫原: KLH conjugated Synthesised phosphopeptide derived from human CREB regulated transcription coactivator 2 around the phosphorylation site of Ser171:TS(p-S)DS

亚型: IgG

纯化方法: affinity purified by Protein A

储存液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed: PubMed

产品介绍

background:
This gene encodes a member of the transducers of regulated cAMP response element-binding protein activity family of transcription coactivators. These proteins promote the transcription of genes targeted by the cAMP response element-binding protein, and therefore play an important role in many cellular processes. Under basal conditions the encoded protein is phosphorylated by AMP-activated protein kinase or the salt-inducible kinases and is sequestered in the cytoplasm. Upon activation by elevated cAMP or calcium, the encoded protein translocates to the nucleus and increases target gene expression. Single nucleotide polymorphisms in this gene may increase the risk of type 2 diabetes. A pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2010].

Function:
Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 Ser-133 phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).

Subunit:
Binds, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1. Arg-314 in the bZIP domain of CREB1 is essential for this interaction. Interaction, via its C-terminal, with TAF4, enhances recruitment of TAF4 to CREB1. Interacts with PPP3CA/calcineurin alpha, SIK2 and 14-3-3 proteins, YWHAB and YWHAG. Interaction with the human T-cell leukemia virus type 1 (HTLV-1) Tax protein is essential for optimal transcription activation by Tax. Interaction with RFWD2/COP1 mediates nuclear export and degradation of CRTC2.

Subcellular Location:
Cytoplasm. Nucleus. Note=Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein. In response to cAMP levels and glucagon, relocated to the nucleus.

Tissue Specificity:
Most abundantly expressed in the thymus. Present in both B and T-lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas.

Post-translational modifications:
Phosphorylation/dephosphorylation states of Ser-171 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation, is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs (SIK1 and SIK2) by LKB1. Both insulin and AMPK increase this phosphorylation of CRTC2 while glucagon suppresses it. Phosphorylation at Ser-274 by MARK2 is induced under low glucose conditions and dephosphorylated in response to glucose influx. Phosphorylation at Ser-274 promotes interaction with 14-3-3 proteins and translocation to the cytoplasm.

Similarity:
Belongs to the TORC family.

SWISS:
Q53ET0

Gene ID:
200186

Database links:

Entrez Gene: 200186 Human

Entrez Gene: 74343 Mouse

Entrez Gene: 310615 Rat

Omim: 608972 Human

SwissProt: Q53ET0 Human

SwissProt: Q3U182 Mouse

SwissProt: Q3LRZ1 Rat

Unigene: 406392 Human

Unigene: 35627 Mouse

Unigene: 13599 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

CREB转录共激活因子TORC2又称葡萄糖关键调控蛋白TORC-2。

产品图片: Sample:
Lane1: Rectal carcinoma (Human) Lysate at 30 ug
Lane2: Heart(Rat) / Colon carcinoma(Human) / Gastric carcinoma(Human) Lysate at 30 ug
Primary: Anti-Phospho-Torc2/Crtc2(Ser171) (bs-3415R) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 73kD
Observed band size : 73kD
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-Phospho-TORC2 (Ser171) antibody (bs-3415R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.