Anti-CXCL2/GRO Beta【科瑞奥博】

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Anti-CXCL2/GRO Beta

货号： bs-1162R 基本售价： 380.0 元规格： 20ul

规格：20ul: 价格：380.00元

规格：50ul: 价格：780.00元

规格：100ul: 价格：1380.00元

规格：200ul: 价格：2200.00元

产品信息

产品编号: bs-1162R

英文名称: CXCL2/GRO Beta

中文名称: 巨噬细胞炎症蛋白2（ GRＯβ）抗体

别名: C-X-C motif chemokine 2; Chemokine (C X C motif) ligand 2; Chemokine, CXC motif, ligand 2; CINC 2a; CINC2a; CINC3; CXC chemokine; CXCL 2; CXCL2; MIP2; MIP 2; MIP-2; Cytokine-induced neutrophil chemoattractant 3; GRO 2; GRO b; GRO protein, beta; Gro-beta; GRO2; GRO2 oncogene; GROb; GRObeta; GRO Beta; Growth regulated protein beta; GROX; Macrophage inflammatory protein 2 alpha; Macrophage inflammatory protein 2; Melanoma growth stimulatory activity beta; MGSA b; MGSA beta; MGSAbeta; MIP 2; MIP 2a; MIP2 alpha; MIP2; MIP2A; MIP2alpha; SCYB 2; Scyb; SCYB2; Small inducible cytokine subfamily B, member 2.

: Specific References  (2)     |     bs-1162R has been referenced in 2 publications.
[IF=3.73] Chen, Lianyu, et al. "Chinese Herbal Medicine Suppresses Invasion-Promoting Capacity of Cancer-Associated Fibroblasts in Pancreatic Cancer." PLOS ONE 9.4 (2014): e96177.  IHC-P ;  Human.
PubMed:24781445
[IF=3.67] Lobach, Alexandra R., and Jack Uetrecht. "Clozapine Promotes the Proliferation of Granulocyte Progenitors in the Bone Marrow Leading to Increased Granulopoiesis and Neutrophilia in Rats." Chemical Research in Toxicology (2014).  IHC-P ;  Rat.
PubMed:24968143

规格价格: 50ul/780元购买 100ul/1380元购买 200ul/2200元购买大包装/询价

说明书: 50ul 100ul 200ul

研究领域: 免疫学转录调节因子

抗体来源: Rabbit

克隆类型: Polyclonal

交叉反应: Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep,

产品应用: WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=0.2ug/test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分子量: 12kDa

细胞定位: 细胞浆分泌型蛋白

性状: Lyophilized or Liquid

浓度: 1mg/ml

免疫原: KLH conjugated synthetic peptide derived from human MIP2:51-107/107

亚型: IgG

纯化方法: affinity purified by Protein A

储存液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed: PubMed

产品介绍

background:
GRO beta is a member of the CXC, or chemokine class. It contains the ELR domain immediately preceding the first cysteine residue near the amino terminus. Other chemokines in this group include IL8, GRO alpha/beta/gamma, mouse KC, ENA78, GCP2, PBP/CTAPIII/beta TG/NAP2. These chemokines act primarily on neutrophils as chemoattractants and activators, including neutrophil degradation with release of myloperoxidase and other enzymes. GRO beta was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. GRO beta is an approximately 8 kDa polypeptide of 73 amino acids. The precursor form of GRO beta consists of 100 amino acids. To generate the mature GRO beta, the precursor cleaves its amino terminal 27 amino acids. GRO beta shows 60% amino acid homology to human GRO alpha and GRO gamma.

Function:
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.

Subcellular Location:
Secreted.

Post-translational modifications:
The N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells.

Similarity:
Belongs to the intercrine alpha (chemokine CxC) family.

SWISS:
P19875

Gene ID:
2920

Database links:

Entrez Gene: 2920Human

Entrez Gene: 20310Mouse

Entrez Gene: 114105Rat

SwissProt: P19875Human

SwissProt: P10889Mouse

SwissProt: P30348Rat

Unigene: 75765Human

Unigene: 10230Rat

Omim: 139110Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片: Sample:
Plasma (Rat) Lysate at 40 ug
Serum (Rat) Lysate at 40 ug
Primary: Anti-CXCL2 (bs-1162R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12 kD
Observed band size: 12 kD
Tissue/cell: human rectal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MIP2/GRO Beta/CXCL2 Polyclonal Antibody, Unconjugated(bs-1162R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse uterus tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MIP2/GRO Beta/CXCL2 Polyclonal Antibody, Unconjugated(bs-1162R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat colitis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MIP2/GRO Beta/CXCL2 Polyclonal Antibody, Unconjugated(bs-1162R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with bs-1162R Antibody at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).