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货号： bs-3263R 基本售价： 1580.0 元 规格： 100ul
产品信息
- 产品编号
- bs-3263R
- 英文名称
- Phospho-MARCKS (Ser152 + Ser156)
- 中文名称
- 磷酸化丙氨酸蛋白激酶C底物Marcks抗体
- 别 名
- MARCKS (phospho S152 + S156); p-MARCKS (phospho S152 + S156); MARCKS (phospho S159 + S163); 80 kDa protein; 80K L; 80K L protein; 80K-L protein; 80KL; 81 kDa protein, light chain; light chain; MACS; MARCKS; MARCS; MARCS_RAT; MGC52672; myristoylated alanine rich C kinase substrate; Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L); Myristoylated alanine rich protein kinase C substrate; Myristoylated alanine-rich C-kinase substrate; Phosphomyristin; PKCSL; PRKCSL; protein kinase C substrate 80 kDa protein light chain; Protein kinase C substrate.
- 规格价格
- 100ul/1580元购买 大包装/询价
- 说 明 书
- 100ul
- 产品类型
- 磷酸化抗体
- 研究领域
- 心血管 免疫学 信号转导 激酶和磷酸酶 细胞骨架
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Cow, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 30kDa
- 细胞定位
- 细胞浆 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthesised phosphopeptide derived from rat MARCKS around the phosphorylation site of Ser152/156 or Ser159/163(human) :RF(p-S)FKK(p-S)FK
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008]
Function:
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
Subcellular Location:
Cytoplasm, cytoskeleton (Probable). Membrane; Lipid-anchor.
Tissue Specificity:
Highest levels found in spleen and brain. Intermediate levels seen in thymus, ovary, lung and heart. Very low levels seen in kidney, skeletal muscle and liver.
Post-translational modifications:
Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
Similarity:
Belongs to the MARCKS family.
SWISS:
P29966
Gene ID:
25603
Database links:Entrez Gene: 4082 Human
Entrez Gene: 17118 Mouse
Entrez Gene: 25603 Rat
Omim: 177061 Human
SwissProt: P29966 Human
SwissProt: P26645 Mouse
SwissProt: P30009 Rat
Unigene: 519909 Human
Unigene: 712721 Human
Unigene: 30059 Mouse
Unigene: 9560 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
MARCKS是一种分子量为32kDa的膜内蛋白,在组织中广泛表达,但主要高表达在脑内的轴突、小直径的树突和树突棘.
- 产品图片
Paraformaldehyde-fixed, paraffin embedded (Rat Cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-MARCKS (Ser152 + Ser156)) Polyclonal Antibody, Unconjugated (bs-3263R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-MARCKS (Ser152 + Ser156)) Polyclonal Antibody, Unconjugated (bs-3263R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.