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货号： bs-3767R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-3767R
- 英文名称
- Hes5
- 中文名称
- 发状分裂相关增强子-5抗体
- 别 名
- Hairy and enhancer of split 5; HES 5; HES5 protein; MGI:104876; HES5_HUMAN.
- Specific References (1) | bs-3767R has been referenced in 1 publications.[IF=2.96] Zhang, Haihua, et al. "Epidermal growth factor promotes proliferation of dermal papilla cells via Notch signaling pathway." Biochimie (2016). WB ; Other Species.PubMed:27109378
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 免疫学 信号转导 转录调节因子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 18kDa
- 细胞定位
- 细胞核
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Hes5:21-120/166
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene encodes a member of a family of basic helix-loop-helix transcriptional repressors. The protein product of this gene, which is activated downstream of the Notch pathway, regulates cell differentiation in multiple tissues. Disruptions in the normal expression of this gene have been associated with developmental diseases and cancer. [provided by RefSeq, Dec 2008]
Function:
Transcriptional repressor of genes that require a bHLH protein for their transcription
Subunit:
Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.
Subcellular Location:
Nucleus.
Tissue Specificity:
Expressed in fetal heart and brain tumors.
Similarity:
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.
SWISS:
Q5TA89
Gene ID:
388585
Database links:Entrez Gene: 388585 Human
Entrez Gene: 15208 Mouse
Entrez Gene: 79225 Rat
SwissProt: Q5TA89 Human
SwissProt: P70120 Mouse
SwissProt: Q03062 Rat
Unigene: 57971 Human
Unigene: 137268 Mouse
Unigene: 22422 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
Kidney (Rat) Lysate at 40 ug
Primary: Anti-alpha smooth muscle Actin (bs-3767R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kD
Observed band size: 20 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Hes5 Polyclonal Antibody, Unconjugated(bs-3767R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining