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货号： bs-3020R 基本售价： 1580.0 元 规格： 100ul
产品信息
- 产品编号
- bs-3020R
- 英文名称
- phospho-p53BP1 (Ser25 + Ser29)
- 中文名称
- 磷酸化p53结合蛋白1抗体
- 别 名
- 53BP1 (phospho S25); p-53BP1 (phospho S25) ;53BP1 (Phospho-Ser25/29); 53BP1 (Phospho-S25/S29); phospho-p53BP1(Ser25/Ser29); P-53BP1 (Ser25/29); 53 BP1; FLJ41424; MGC138366; p202; p53 binding protein 1; p53 BP1; p53BP1; TP53 BP1; TP53BP1; Tumor protein 53 binding protein 1; Tumor protein p53 binding protein 1; Tumor suppressor p53 binding protein 1; TP53B_HUMAN; tumor protein 53-binding protein, 1.
- Specific References (1) | bs-3020R has been referenced in 1 publications.[IF=4.60] Cai, Zhiqiang, et al. "Oncogenic miR-17/20a Forms a Positive Feed-forward Loop with the p53 Kinase DAPK3 to Promote Tumorigenesis." Journal of Biological Chemistry (2015): jbc-M115. WB ; Human.PubMed:26117336
- 规格价格
- 100ul/1580元购买 大包装/询价
- 说 明 书
- 100ul
- 产品类型
- 磷酸化抗体
- 研究领域
- 肿瘤 细胞生物 染色质和核信号 信号转导 激酶和磷酸酶 结合蛋白 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 213kDa
- 细胞定位
- 细胞核
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated Synthesised phosphopeptide derived from human p53BP1 around the phosphorylation site of Ser25/29:ED(p-S)QPE(p-S)QV
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
p53 binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double strand breaks. This also suggests a role in DNA repair, maintaining genomic stability.
Function:
May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
Subunit:
Interacts with IFI202A. Binds to the central domain of p53/TP53. May form homooligomers. Interacts with DCLRE1C. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on Ser-139. Interacts with histone H4 that has been dimethylated at Lys-20 (H4K20me2). Has low affinity for histone H4 containing monomethylated Lys-20 (H4K20me1). Does not bind histone H4 containing unmethylated or trimethylated Lys-20 (H4K20me3). Has low affinity for histone H3 that has been dimethylated on Lys-79. Has very low affinity for histone H3 that has been monomethylated on Lys-79 (in vitro). Does not bind unmethylated histone H3. Interacts with MUM1/EXPAND1. Interacts with CHEK2; modulates CHEK2 phosphorylation at Thr-68 in response to infrared. Interacts with MSL1; this interaction may be required for MSL1 DNA repair activity, but not for histone acetyltransferase activity.
Subcellular Location:
Nucleus. Chromosome; centromere; kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at Lys-20 is required for efficient localization to double strand breaks.
Post-translational modifications:
Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
DISEASE:
Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
Similarity:
Contains 2 BRCT domains.
SWISS:
Q12888
Gene ID:
7158
Database links:Entrez Gene: 7158 Human
Entrez Gene: 27223 Mouse
Omim: 605230 Human
SwissProt: Q12888 Human
SwissProt: P70399 Mouse
Unigene: 440968 Human
Unigene: 383499 Mouse
Unigene: 481841 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
p53结合蛋白1(53BP1)是细胞周期检查点激酶蛋白，是新近发现的DNA损伤修复通路中两个非常重要的蛋白激酶MDC1和53BP1蛋白之一,目前多用于肿瘤方面的研究。
- 产品图片
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-p53BP1 (Ser25 + Ser29)) Polyclonal Antibody, Unconjugated (bs-3020R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-p53BP1 (Ser25 + Ser29)) Polyclonal Antibody, Unconjugated (bs-3020R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.