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货号： bs-2652R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-2652R
- 英文名称
- SPHK1
- 中文名称
- 鞘氨醇激酶1抗体
- 别 名
- sphingosine kinase 1; SK 1; SK1; SPHK 1; SPHK; SPK; SPK 1; SPK1; Sphk1; SPHK1_HUMAN.
- Specific References (2) | bs-2652R has been referenced in 2 publications.[IF=4.29] Tran, Hai B., et al. "Cigarette smoke inhibits efferocytosis via deregulation of sphingosine kinase signaling: reversal with exogenous S1P and the S1P analogue FTY720." Journal of Leukocyte Biology (2016): jlb-3A1015. Human.PubMed:26792820[IF=3.53] Tani, Kohsuke, Keiji Tabuchi, and Akira Hara. "Hair Cell Loss Induced by Sphingosine and a Sphingosine Kinase Inhibitor in the Rat Cochlea."Neurotoxicity research 29.1 (2016): 35-46. WB, IF(ICC) ; Rat.PubMed:26472207
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 信号转导 细胞凋亡 激酶和磷酸酶 细胞分化
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Cow, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 42-52kDa
- 细胞定位
- 细胞核 细胞浆 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human SPHK1:1-100/384
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Function:
Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.
Tissue Specificity:
Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.
Similarity:
Contains 1 DAGKc domain.
SWISS:
Q9NYA1
Gene ID:
8877
Database links:Entrez Gene: 8877 Human
Entrez Gene: 20698 Mouse
Entrez Gene: 170897 Rat
Omim: 603730 Human
SwissProt: Q9NYA1 Human
SwissProt: Q8CI15 Mouse
SwissProt: Q91V26 Rat
Unigene: 68061 Human
Unigene: 20944 Mouse
Unigene: 18522 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
SPHK1参与鞘氨醇代谢、G蛋白偶联受体蛋白信号转导途径，有抗细胞凋亡的作用，可被蛋白激酶C激活，参与细胞内信号转导级联、钙介导信号转导、调控细胞生长迁移、调控血管生成及成纤维细胞增殖。
- 产品图片
Sample: Heart (Mouse) Lysate at 30 ug
Primary: Anti- SPHK1 (bs-2652R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPHK1) Polyclonal Antibody, Unconjugated (bs-2652R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.