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货号： bs-0084R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0084R
- 英文名称
- Caspase-6 subunit p18
- 中文名称
- 半胱胺酸蛋白酶蛋白-6抗体
- 别 名
- Caspase-6; Caspase-6 p18; cleaved Caspase-6 p18; Apoptosis Related Cysteine Protease; Apoptotic cysteine protease MCH2; Apoptotic protease MCH 2; Apoptotic protease MCH2; Casp 6; Casp6; Caspase 6 apoptosis related cysteine protease; Caspase 6 precursor; Caspase6; Human cysteine protease Mch2 isoform alpha; Mch 2; Mch2; CASP6_HUMAN; caspase-6 isoform alpha precursor.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 细胞生物 神经生物学 信号转导 细胞凋亡
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 18/33kDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Caspase-6 subunit p18:27-130/293
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in two transcript variants that encode different isoforms. [provided by RefSeq, Jul 2008]
Function:
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
Subunit:
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce.
Subcellular Location:
Cytoplasm.
Post-translational modifications:
Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
Similarity:
Belongs to the peptidase C14A family.
SWISS:
P55212
Gene ID:
839
Database links:Entrez Gene: 839 Human
Entrez Gene: 12368 Mouse
Entrez Gene: 83584 Rat
Omim: 601532 Human
SwissProt: P55212 Human
SwissProt: O08738 Mouse
SwissProt: O35397 Rat
Unigene: 654616 Human
Unigene: 281379 Mouse
Unigene: 88160 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Caspase-6 与Caspase-3有38%的同源性，属胱氨酸-天冬氨酸蛋白酶家族。在凋亡执行阶段起中心作用，可被Caspase-7、-8、-10剪切。它是唯一一个可以剪切核纤层蛋白的Caspase。
- 产品图片
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-Caspase-6 subunit p18 (bs-0084R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18/33 kD
Observed band size: 33 kD
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-Caspase-6 subunit p18 (bs-0084R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18/33 kD
Observed band size: 33 kD
Tissue/cell: Human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- Caspase-6 Polyclonal Antibody, Unconjugated(bs-0084R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Caspase-6 Polyclonal Antibody, Unconjugated(bs-0084R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
Tissue/cell: mouse intestine tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Caspase-6 Polyclonal Antibody, Unconjugated(bs-0084R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei