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货号： bs-2633R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-2633R
- 英文名称
- IL33
- 中文名称
- 白介素33抗体
- 别 名
- Interleukin-33/Nuclear factor from high endothelial venules; IL33; C9orf26; CHROMOSOME 9 OPEN READING FRAME 26; DKFZp586H0523; DVS27; DVS27 related protein; IL 1F11; IL 33; IL-33; IL1F11; Interleukin 1 family member 11; Interleukin 33; INTERLEUKIN 33 NFHEV; Interleukin 33 precursor; Interleukin33; NF HEV; NFEHEV; NFHEV; Nuclear factor from high endothelial venules; RP11 575C20.2; IL33_MOUSE; Interleukin-33; Interleukin-1 family member 11; IL-1F11; NF-HEV.
- Specific References (3) | bs-2633R has been referenced in 3 publications.[IF=0.94] Demirtas, Levent, et al. "The possible role of interleukin-33 as a new player in the pathogenesis of contrast-induced nephropathy in diabetic rats." Renal Failure (2016): 1-9. IHC-P ; Rat.PubMed:27055881[IF=1.89] Liu, Kai, and Qing-Hong Cheng. "Interleukin-33 plays an important role in sepsis-induced acute lung injury in mice." International Journal of Clinical and Experimental Pathology 9.5 (2016): 5025-5033. WB ; Mouse.PubMed:0[IF=2.55] Chen, Baiwen, et al. "Effects of 1, 25-dihydroxyvitamin D3 in an ovalbumin-induced allergic rhinitis model." International Immunopharmacology 47 (2017): 182-189. IHC-P ; Mouse.PubMed:28412624
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 免疫学 细胞表面分子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Mouse, Rat,
- 产品应用
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 30kDa
- 细胞定位
- 细胞核 细胞浆 分泌型蛋白
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from mouse IL-33:185-264/264
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
IL33 is a cytokine which belongs to the IL-1 superfamily, and it induces helper T cells to produce type 2 cytokines. This cytokine was previously named NF-HEV nuclear factor (NF) in high endothelial venules (HEVs), as it was originally identified in these specialized cells.
IL33 mediates its biological effects by interacting with the receptors ST2 and IL-1 Receptor Accessory Protein, activating intracellular molecules in the NF-kappaB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-4, IL-5 and IL-13) from polarized Th2 cells. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the the severe pathological changes observed in mucosal organs following administration of IL33.
Function:
Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Acts as a chemoattractant tor Th2 cells, and may function as an alarmin, that amplifies immune responses during tissue injury. [FUNCTION] In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation.
Subunit:
Forms a 1:1:1 heterotrimeric complex with its primary high-affinity receptor IL1RL1 and the coreceptor IL1RAP.
Subcellular Location:
Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Note=Associates with heterochromatin and mitotic chromosomes. Translocation from the nucleus occurs upon biomechanical strain, depends on an intact microtubule network, and is ATP-dependent.
Tissue Specificity:
Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.
Post-translational modifications:
Proteolytically converted to a mature form by CASP1 in vitro and calpains in vivo. Caspase-mediated proteolysis, once thought to activate IL33, rather acts as a switch to dampen its activity. Cathepsin G and elastase can cleave IL33 and generate highly active forms in activated neutrophils (IL-33(95-270), IL-33(99-270) and IL-33(109-270)). Proteolysis is not strictly required for biological activity.
Similarity:
Belongs to the IL-1 family. Highly divergent.
SWISS:
Q8BVZ5
Gene ID:
77125
Database links:Entrez Gene: 90865 Human
Entrez Gene: 77125 Mouse
Omim: 608678 Human
SwissProt: O95760 Human
SwissProt: Q8BVZ5 Mouse
Unigene: 731660 Human
Unigene: 182359 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
IL-33是最近发现的一种前炎症细胞因子,IL-1家族的细胞因子,IL-33分布广泛,在胃、肺、脊髓、脑和皮肤中高表达它结合IL-1受体家族成员ST2,活化NF-kappa B和MAPK信号通路,促进Th2细胞因子的产生,参与多种炎症与免疫反应过程. 作为核因子则能定位干细胞核内,起到转录抑制作用。
- 产品图片
Tissue/cell: rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IL33 Polyclonal Antibody, Unconjugated(bs-2633R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL33) Polyclonal Antibody, Unconjugated (bs-2633R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.