| 货号 | 3759T |
| 同种亚型 | Rabbit |
| 反应种属 | Human, Mouse |
| 应用 | WB |
| 目标/特异性 | SHP-1 (C14H6) Rabbit mAb detects endogenous level of total SHP-1 protein. |
| 使用方法 | Western Blotting (1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain (1). SHP-1 removes phosphates from target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EpoR) to negatively regulate hematopoietic growth (2). Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation (3). Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R β chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation (4). Because SHP-1 downregulates various proliferation pathways, SHP-1 is considered a potential tumor suppressor and angiogenesis regulator (5,6). |
| 存放说明 | Upon receipt of the kit, components must immediately be placed at their appropriate storage temperature in an upright position: Ambient,? 癈 to 8癈, -30 ?to 10 癈, and -85 ?to -75 癈. |
| 计算分子量 | 68 |
| 参考文献 | 1 . Yi, T.L. et al. (1992) Mol Cell Biol 12, 836-46. 2 . Yi, T. et al. (1995) Blood 85, 87-95. 3 . Keilhack, H. et al. (2001) J Cell Biol 152, 325-34. 4 . Yi, T. et al. (1993) Mol Cell Biol 13, 7577-86. 5 . Wu, C. et al. (2003) Gene 306, 1-12. 6 . Bhattacharya, R. et al. (2008) J Mol Signal 3, 8. |
Western blot analysis of extracts from Jurkat, 32D and BaF3 cells using SHP-1 (C14H6) Rabbit mAb. |
