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货号： bs-1007R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1007R
- 英文名称
- Dopamine Receptor D1
- 中文名称
- 多巴胺受体D1抗体
- 别 名
- DRD1; dopamine D1 receptor; D(1A) dopamine receptor; D1A dopamine receptor; Dopamine D1Receptors; D1DR; DADR; Dopamine Receptor D1; DR D1; DR D1A; DRD 1; DRD1 receptor; DRD1; DRD1A; DRD1_HUMAN; D(1A) dopamine receptor; DRD 1A; DRD1.
- Specific References (3) | bs-1007R has been referenced in 3 publications.[IF=2.35] Xu, Jiao-jiao, et al. "Dopamine D1 receptor activation induces dehydroepiandrosterone sulfotransferase (SULT2A1) in HepG2 cells." Acta Pharmacologica Sinica (2014). WB ; Human.PubMed:24909515[IF=3.26] Salgado, R., et al. "Perfluorooctane sulfonate (PFOS) exposure could modify the dopaminergic system in several limbic brain regions." Toxicology Letters(2015). WB ; Rat.PubMed:26529483[IF=8.46] Zhang, Q. B., et al. "Moderate swimming suppressed the growth and metastasis of the transplanted liver cancer in mice model: with reference to nervous system." Oncogene (2016). WB ; Human.PubMed:26686088
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 免疫学 神经生物学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 50kDa
- 细胞定位
- 细胞浆 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human DRD1:101-200/446 <Extracellular>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq, Jul 2008]
Function:
Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
Subunit:
Interacts with DNAJC14 via its C-terminus. Interacts with DRD1IP.
Subcellular Location:
Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
Tissue Specificity:
Detected in caudate, nucleus accumbens and in the olfactory tubercle.
Similarity:
Belongs to the G-protein coupled receptor 1 family.
SWISS:
P21728
Gene ID:
1812
Database links:Entrez Gene: 1812Human
Entrez Gene: 13488Mouse
Entrez Gene: 24316Rat
Omim: 126449Human
SwissProt: P21728Human
SwissProt: Q61616Mouse
SwissProt: P18901Rat
Unigene: 2624Human
Unigene: 54161Mouse
Unigene: 24039Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
多巴胺受体D1在运动协调方面起重要作用，该受体的缺失对黑质多巴胺能神经元的影响程度虽没临床帕金森病（PD）严重，但仍可加速多巴胺能神经元发生退行性改变.该蛋白目前主要用于神经退行性改变的研究。
- 产品图片
Sample: 293T Cell Lysate at 40 ug
Primary: Anti- Dopamine Receptor D1 (bs-1007R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 50 kD
Observed band size: 48 kD
Sample: Cerebrum (Mouse)Lysate at 40 ug
Primary: Anti- Dopamine Receptor D1 (bs-1000R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 50 kD
Observed band size: 48 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DRD1 Polyclonal Antibody, Unconjugated(bs-1007R) 1:300, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: HUVEC cells(blue). Primary Antibody:Rabbit Anti-CD31 antibody(bs-0468R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.ProtocolThe cells were fixed with 2% paraformaldehyde (10 min) .Primary antibody (bs-0468R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed
Blank control: Hela(blue).
Primary Antibody:Rabbit Anti- Dopamine Receptor D1 antibody(bs-1007R), Dilution: 1レg in 100 レL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Antibody (bs-1007R, 1レg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-1007R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.