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货号： bs-2503R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-2503R
- 英文名称
- eEF1A1
- 中文名称
- 翻译延伸因子EF-1a抗体
- 别 名
- Elongation Factor 1, Alpha; EF1 Alpha; eukaryotic translation elongation factor 1 alpha 1; CCS3; eEF1A-1; EF-Tu; EF1A; Elongation factor Tu; GRAF-1EF; PTI1; elongation factor 1-alpha 1; EEF-1; EEF1A; Eef1a2; Eef1a2l1; EF1A; MGC125172; SI; Eef1a1; EF1A1_HUMAN.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 免疫学 信号转导 细胞凋亡 转录调节因子 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 50kDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human EF1 alpha:131-230/462
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
eEF1A1 is an isoform of the alpha subunit of the elongation factor 1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. It promotes the GTP dependent binding of aminoacyl tRNA to the A site of ribosomes during protein biosynthesis. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome (referenced from entrez gene and swissprot).
Function:
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle
Post-translational modifications:
ISGylated.
Similarity:
Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
SWISS:
P68104
Gene ID:
1915
Database links:Entrez Gene: 1915Human
Entrez Gene: 13627Mouse
Entrez Gene: 171361Rat
Omim: 130590Human
SwissProt: P68104Human
SwissProt: P10126Mouse
SwissProt: P62630Rat
Unigene: 520703Human
Unigene: 535192Human
Unigene: 586423Human
Unigene: 695681Human
Unigene: 713109Human
Unigene: 138471Mouse
Unigene: 335315Mouse
Unigene: 360075Mouse
Unigene: 391071Mouse
Unigene: 468942Mouse
Unigene: 144660Rat
Unigene: 965Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
肽链延长因子1A-1(peptide elongation factor 1A-1,eEF1A-1)是真核细胞蛋白翻译过程中肽链延长复合物的重要构成成分,具有调节蛋白合成及促发凋亡等多种作用.
- 产品图片
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-eEF1A1 Polyclonal Antibody, Unconjugated(bs-2503R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Mouse embryos; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-EF1a/eEF1A1 Polyclonal Antibody, Unconjugated(bs-2503R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining