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货号： bs-2437R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-2437R
- 英文名称
- ENPP2
- 中文名称
- 自分泌运动因子抗体
- 别 名
- Autotaxin; Ectonucleotide pyrophosphatase/phosphodiesterase family member 2; ATX; ATX X; ATX-X; Autotaxin; E NPP 2; ENPP2; Ectonucleotide pyrophosphatase/phosphodiesterase 2; Extracellular lysophospholipase D; FLJ26803; LysoPLD; NPP2; PD IALPHA; PD-IALPHA; PDNP2; Phosphodiesterase I alpha; Phosphodiesterase I/nucleotide pyrophosphatase 2; Plasma lysophospholipase D; ENPP2_HUMAN.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 免疫学 神经生物学 信号转导 细胞凋亡 激酶和磷酸酶
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 95kDa
- 细胞定位
- 分泌型蛋白
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human ENPP2:601-700/915
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5 end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]
Function:
Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor.
Subcellular Location:
Secreted. Note=Secreted by most body fluids including serum and CSF. Also by adipocytes and numerous cancer cells.
Tissue Specificity:
Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues.
Post-translational modifications:
N-glycosylation, but not furin-cleavage, plays a critical role on secretion and on lysoPLD activity.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Similarity:
Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
Contains 2 SMB (somatomedin-B) domains.
SWISS:
Q13822
Gene ID:
5168
Database links:Entrez Gene: 5168Human
Entrez Gene: 18606Mouse
Omim: 601060Human
SwissProt: Q13822Human
SwissProt: Q9R1E6Mouse
Unigene: 190977Human
Unigene: 250256Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
ATX是一个分泌型糖蛋白,具有磷酸二酯酶（PDE）活性，ATX是胞外焦磷酸酶／磷酸二酯酶（ENPP）家族的一员。ATX还具有溶血磷脂酶D（lysoPLD）活性，能够以溶血磷脂酰胆碱（lysophosphatidylcboline，LPC）为底物催化生成溶血磷脂酸（lysophosphatidic acid，LPA）。
ATX在很多肿瘤细胞中都有高表达，在肿瘤的发生、发展过程中有着重要作用。此外，ATX在神经系统、免疫系统中也发挥重要作用。
- 产品图片
Sample:
DU145(Human) Cell Lysate at 30 ug
Primary: Anti-Anei-ENPP2 (bs-2437R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kD
Observed band size: 115 kD
Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Anei-ATX/Autotaxin Polyclonal Antibody, Unconjugated(bs-2437R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining