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货号： bs-1913R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1913R
- 英文名称
- MMP8
- 中文名称
- 基质金属蛋白酶-8/胶原酶2抗体
- 别 名
- Matrilysin/matrix metalloproteinases-8; CLG 1; CLG1; Collagenase II; Collagenase 2; Collagenase neutrophil; HNC; Matrix metalloprotease 8; MMP8; MMP 8; MMP-8; Neutrophil collagenase; PMNL CL; PMNL collagenase; PMNLCL; neutrophil collagenase preproprotein; MMP8_HUMAN; Neutrophil collagenase;Matrix metalloproteinase-8; PMNL-CL.
- Specific References (2) | bs-1913R has been referenced in 2 publications.[IF=2.35] Zhang, Chao-ying, et al. "Hydrogen sulfide suppresses the expression of MMP-8, MMP-13, and TIMP-1 in left ventricles of rats with cardiac volume overload." Acta Pharmacologica Sinica (2013). Rat.PubMed:23974514[IF=3.27] Oralova, V., et al. "Osteogenic Potential of the Transcription Factor c-MYB." Calcified Tissue International: 1-12. WB ; Mouse.PubMed:28012106
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 神经生物学 信号转导
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-F=1:400-800 Flow-Cyt=1ug/test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 38kDa
- 细胞定位
- 细胞浆 细胞外基质 分泌型蛋白
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human MMP8:241-340/467
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]
Function:
Can degrade fibrillar type I, II, and III collagens.
Subcellular Location:
Cytoplasmic granule. Secreted, extracellular space, extracellular matrix (Probable). Note=Stored in intracellular granules.
Tissue Specificity:
Neutrophils.
Similarity:
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
SWISS:
P22894
Gene ID:
4317
Database links:Entrez Gene: 4317 Human
Entrez Gene: 17394 Mouse
Entrez Gene: 63849 Rat
Omim: 120355 Human
SwissProt: P22894 Human
SwissProt: O70138 Mouse
SwissProt: O88766 Rat
Unigene: 161839 Human
Unigene: 16415 Mouse
Unigene: 44474 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample: Bone (Mouse) Lysate at 30 ug
Primary: Anti-MMP8 (bs-1913R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 38 kD
Observed band size: 38 kD
Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-MMP8 /AF647 Conjugated antibody (bs-1913R-AF647)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-AF647 .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.