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货号： bs-1840R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1840R
- 英文名称
- Lamin B
- 中文名称
- 核纤层蛋白B抗体(细胞核膜标志物)
- 别 名
- lamin B1; LMB1; LMN; LMN2; LMNB 1; LMNB; LMNB1; MGC111419; LMNB1_HUMAN; Lamin-B1.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 细胞生物 染色质和核信号 信号转导 细胞凋亡 细胞类型标志物
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Pig, Cow,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 64kDa
- 细胞定位
- 细胞核
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Lamin B:361-460/586
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). [provided by RefSeq, Oct 2010].
Function:
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Subunit:
Homodimer. Interacts with lamin-associated polypeptides IA, IB and 2.
Subcellular Location:
Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side.
Post-translational modifications:
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
DISEASE:
Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P20700
Gene ID:
4001
Database links:Entrez Gene: 396223 Chicken
Entrez Gene: 4001 Human
Entrez Gene: 16906 Mouse
Entrez Gene: 116685 Rat
Omim: 150340 Human
SwissProt: P14731 Chicken
SwissProt: P20700 Human
SwissProt: P14733 Mouse
SwissProt: P70615 Rat
Unigene: 89497 Human
Unigene: 4105 Mouse
Unigene: 11362 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
核膜标志物（Nuclear Envelope Marker）
核纤层蛋白(Lamin) 是紧贴核内膜的一层厚度为20～50nm的纤维蛋白层或纤维网络。核纤层与细胞质骨架、核骨架连成一个整体，一般认为核纤层将核被膜和染色质提供了结构支架。有学者研究认为：lamin蛋白与细胞凋亡及衰老有关联，它包括：核纤层蛋白A、核纤层蛋白B、核纤层蛋白C几个不同亚型的蛋白。
- 产品图片
Sample: Brain(Rat) lysate at 30ug;
Primary: Anti-Lamin B (bs-1840R) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 64kD
Observed band size : 64kD
Blank control (Black line):Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-Lamin B antibody (bs-1840R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.