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货号： bs-1611R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1611R
- 英文名称
- Cathepsin K
- 中文名称
- 组织蛋白酶K抗体
- 别 名
- Cathepsin K; Procathepsin K; Cathepsin K precursor; Cathepsin O; Cathepsin O1; Cathepsin O2; Cathepsin X; CathepsinK; CTS02; CTSK; CTSK protein; CTSO; CTSO1; CTSO2; MGC23107; PKND; PYCD; Pycnodysostosis; cath-K; CK.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 合成与降解
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Guinea Pig,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 36kDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Cathepsin K:112-210/329
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. This gene may be subject to RNA editing. [provided by RefSeq, Jul 2008].
Function:
Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.
Subunit:
Belongs to the peptidase C1 family.
Subcellular Location:
Lysosome.
Tissue Specificity:
Tissue specificityPredominantly expressed in osteclasts (bones).
DISEASE:
Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature.
Similarity:
Belongs to the peptidase C1 family.
SWISS:
P43235
Gene ID:
1513
Database links:Entrez Gene: 1513 Human
Entrez Gene: 13038 Mouse
Entrez Gene: 29175 Rat
Omim: 601105 Human
SwissProt: P43235 Human
SwissProt: P55097 Mouse
SwissProt: O35186 Rat
Unigene: 632466 Human
Unigene: 272085 Mouse
Unigene: 5598 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
合成与降解（Synthesis and Degradation）
组织蛋白酶K(cathepsinK)是半胱氨酸蛋白酶家族的成员,它和组织蛋白酶S、L和B有很高的同源性。
在心血管方面：组织蛋白酶K参与心血管的很多病理生理过程，在动脉硬化、血管重塑与再生。也是动脉瘤生长、破裂、主动脉辩狭窄进程中主要的胶原酶之一。在骨与关节方面：组织蛋白酶K主要位于破骨细胞，是涉及骨质再吸收的主要酶，与骨质疏松与脱钙密切相关。
- 产品图片
Sample: Bone (Rat) Lysate at 40 ug
Primary: Anti-Cathepsin K (bs-1611R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 34 kD
Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cathepsin K Polyclonal Antibody, Unconjugated(bs-1611R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining