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货号： bs-2921R-HRP 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-2921R-HRP
- 英文名称
- Anti-PAR3/HRP
- 中文名称
- 辣根过氧化物酶标记的非典型蛋白激酶C特定相互作用蛋白抗体
- 别 名
- par-3; agouti signaling protein; ASIP; Atypical PKC isotype specific interacting protein; CTCL tumor antigen se2-5; PAR3 alpha; par3; par 3; par-3; PARD3; Partitioning-defective 3 homolog.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 研究领域
- 肿瘤 免疫学 信号转导 转录调节因子 细胞粘附分子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit,
- 产品应用
- WB=1:50-200 ELISA=1:100-1000 IHC-F=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 149kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human PAR3.
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
PAR3 is an adapter protein involved in asymmetrical cell division and cell polarization processes. It seems to play a central role in the formation of epithelial tight junctions. Its association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP bound Rho small GTPases to atypical protein kinase C proteins.
Function:
Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.
Subunit:
Interacts (via PDZ 1 domain) with F11R/JAM1, PARD6A and PARD6B. Isoform 2, but not at least isoform 3 interacts with PRKCZ.Interacts with PRCKI and CDH5. Interacts (via PDZ 3 domain) with PTEN (via C-terminus) (By similarity). Part of a complex with PARD6A or PARD6B, PRKCI or PRKCZ and CDC42 or RAC1. Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1,INADL/PATJ and PARD3/PAR3. Interacts with LIMK2, AURKA and AURKB.Component of the Par polarity complex, composed of at least phosphorylated PRKCZ, PARD3 and TIAM1. Interacts with ECT2 and FBF1.
Subcellular Location:
Endomembrane system. Cell junction. Cell junction, tight junction. Cell membrane. Cytoplasm, cell cortex (By similarity). Cytoplasm, cytoskeleton. Note=Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II (By similarity). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
Tissue Specificity:
Widely expressed.
Post-translational modifications:
Phosphorylated by PRKCZ. EGF-induced Tyr-1127 phosphorylation mediates dissociation from LIMK2.
Phosphorylation by AURKA at Ser-962 is required for the normal establishment of neuronal polarity.
Similarity:
Belongs to the PAR3 family.
Contains 3 PDZ (DHR) domains.
Database links:Entrez Gene: 56288 Human
Entrez Gene: 93742 Mouse
Entrez Gene: 81918 Rat
Entrez Gene: 101110782 Sheep
Omim: 606745 Human
SwissProt: Q8TEW0 Human
SwissProt: Q99NH2 Mouse
SwissProt: Q9Z340 Rat
Unigene: 131489 Human
Unigene: 299254 Mouse
Unigene: 31803 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.