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货号： bs-0677R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0677R
- 英文名称
- Angiopoietin 2
- 中文名称
- 血管生成素2抗体
- 别 名
- AGPT 2; AGPT-2; Agpt2; ANG 2; ANG2; ANG-2; Angiopoietin 2a; Angiopoietin 2B; Angiopoietin2; Angiopoietin-2; ANGPT 2; ANGPT2; Tie2 ligand; ANGP2_HUMAN.
- Specific References (3) | bs-0677R has been referenced in 3 publications.[IF=2.14] Lv, Ming-ming, Xin-dong Fan, and Li-xin Su. "Is a Swine Model of Arteriovenous Malformation Suitable for Human Extracranial Arteriovenous Malformation? A Preliminary Study." Cardiovascular and interventional radiology (2013): 1-7. IHC-P ;PubMed:23652418[IF=2.84] Zhao, Qiuchen, et al. "Intranasal administration of Human umbilical cord mesenchymal stem cells-conditioned medium enhances vascular remodeling after stroke." Brain Research (2015). WB ; Rat.PubMed:26279113[IF=0.00] Du, Weiyan, et al. "The Expression and Significance of Angiopoietin-2 and Glucose Regulated Protein78 in Colorectal Cancer." Cancer Cell Research (2016). IHC-P ; Human.PubMed:0
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 心血管 生长因子和激素
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Pig,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 53kDa
- 细胞定位
- 分泌型蛋白
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Angiopoietin 2:401-496/496
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function:
Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
Subunit:
Interacts with TEK/TIE2, competing for the same binding site as ANGPT1.
Subcellular Location:
Secreted.
Similarity:
Contains 1 fibrinogen C-terminal domain.
SWISS:
O15123
Gene ID:
285
Database links:Entrez Gene: 285Human
Entrez Gene: 11601Mouse
Entrez Gene: 89805Rat
Omim: 601922Human
SwissProt: O15123Human
SwissProt: O35608Mouse
SwissProt: O35462Rat
Unigene: 583870Human
Unigene: 439874Mouse
Unigene: 138360Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
血管生成素2(angiopoietin-2, Ang-2)是Ang家族的成员之一,是特异性血管生成刺激因子,也是恶性肿瘤早期的标志分子.
- 产品图片
Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-Angiopoietin 2 (bs-0677R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Tissue/cell: human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Ang2 Polyclonal Antibody, Unconjugated(bs-0677R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining