Anti-Annexin A1【科瑞奥博】

产品中心

当前位置：首页>产品中心

Anti-Annexin A1

货号： bs-1562R 基本售价： 780.0 元规格： 50ul

规格：50ul: 价格：780.00元

规格：100ul: 价格：1380.00元

规格：200ul: 价格：2200.00元

产品信息

产品编号: bs-1562R

英文名称: Annexin A1

中文名称: 膜粘连蛋白A1抗体

别名: Annexin A1; Annexin I; AnnexinA1; AnnexinI; ANX 1; ANX A1; ANX1; ANXA 1; ANXA1; ANXA1 protein; Calpactin II; CalpactinII; Chromobindin 9; Chromobindin9; HGNC:533; Lipocortin 1; Lipocortin I; Lipocortin1; LipocortinI; LPC 1; LPC1; p35; Phospholipase A2 inhibitory protein; ANXA1_HUMAN.

: Specific References  (2)     |     bs-1562R has been referenced in 2 publications.
[IF=3.35] Li, Yangguang, et al. "Glycoproteomic analysis of tissues from patients with colon cancer using lectin microarrays and nanoLC-MS/MS." Mol. BioSyst. (2013).  WB ;  Human.
PubMed:23567825
[IF=2.46] Wang, Zhong-Chao, et al. "2D-DIGE proteomic analysis of changes in estrogen/progesterone-induced rat breast hyperplasia upon treatment with the Mongolian remedy RuXian-I." Molecules 16.4 (2011): 3048-3065.  Rat.
PubMed:21478820

规格价格: 50ul/780元购买 100ul/1380元购买 200ul/2200元购买大包装/询价

说明书: 50ul 100ul 200ul

研究领域: 肿瘤

抗体来源: Rabbit

克隆类型: Polyclonal

交叉反应: Human, Mouse, Rat, Pig, Cow, Horse, Rabbit,

产品应用: WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分子量: 39kDa

细胞定位: 细胞核细胞浆细胞膜

性状: Lyophilized or Liquid

浓度: 1mg/ml

免疫原: KLH conjugated synthetic peptide derived from human Annexin A1:281-346/346

亚型: IgG

纯化方法: affinity purified by Protein A

储存液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed: PubMed

产品介绍

background:
This gene encodes a protein that belongs to a family of Ca(2+) dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti inflammatory activity.

Function:
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.

Subunit:
Homodimer in placenta (20%); linked by transglutamylation. Interacts with DYSF (By similarity).

Subcellular Location:
Nucleus. Cytoplasm. Cell projection, cilium. Basolateral cell membrane. Note=Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium. Found in the cytoplasm of type II pneumocytes and alveolar macrophages.

Post-translational modifications:
Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.

Similarity:
Belongs to the annexin family.
Contains 4 annexin repeats.

SWISS:
P04083

Gene ID:
301

Database links:

Entrez Gene: 301Human

Entrez Gene: 16952Mouse

Entrez Gene: 25380Rat

Omim: 151690Human

SwissProt: P04083Human

SwissProt: P10107Mouse

SwissProt: P07150Rat

Unigene: 494173Human

Unigene: 248360Mouse

Unigene: 1792Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片: Sample:
Lane1: Kidney(Rat) Lysate at 30 ug
Lane2: Brain(Rat) Lysate at 30 ug
Primary: Anti-Annexin A1 (bs-1562R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 39kD
Observed band size : 40kD
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37鈩� for 20 min;
Incubation: Anti-Annexin A1 Polyclonal Antibody, Unconjugated(bs-1562R) 1:200, overnight at 4掳C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining