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货号： bs-1382R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1382R
- 英文名称
- SNAI2
- 中文名称
- 锌指转录因子Slug抗体
- 别 名
- SLUG; Neural crest transcription factor Slug; Protein snail homolog 2; Slug (chicken homolog) zinc finger protein; Slug homolog zinc finger protein; Slug zinc finger protein; SLUGH 1; SLUGH; SLUGH1; SNAI 2; SNAI2; SNAI-2; SNAI2_HUMAN; Snail 2; Snail homolog 2; Snail2; Snail-2; WS 2D; WS2D; Zinc finger protein SLUG; Zinc finger protein SNAI2.
- Specific References (3) | bs-1382R has been referenced in 3 publications.[IF=3.02] Kong, Lingxin, et al. "Overexpression of SDF-1 activates the NF-κB pathway to induce epithelial to mesenchymal transition and cancer stem cell-like phenotypes of breast cancer cells." International Journal of Oncology. other ; Human.PubMed:26782945[IF=2.31] Liu, Baolin, et al. "Overexpression of Livin promotes migration and invasion of colorectal cancer cells by induction of epithelial–mesenchymal transition via NF-κB activation." OncoTargets and Therapy 9 (2016): 1011-1021. WB ; Human.PubMed:27013894[IF=5.02] Bai, J. W., et al. "The zinc-finger transcriptional factor Slug transcriptionally downregulates ERα by recruiting lysine-specific demethylase 1 in human breast cancer." Oncogenesis 6.5 (2017): e330. IHC-P ; Human.PubMed:28481366
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 免疫学 神经生物学 信号转导 转录调节因子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 30kDa
- 细胞定位
- 细胞核
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Slug:5-120/268
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq, Jul 2008].
Function:
Transcriptional repressor. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occluding (OCLN) and subsequent oncogenic transformation of epithelial cells.
Subunit:
Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains).
Subcellular Location:
Nucleus. Cytoplasm. Note=Observed in discrete foci in interphase nuclei. These nuclear foci do not overlap with the nucleoli, the SP100 and the HP1 heterochromatin or the coiled body, suggesting SNAI2 is associated with active transcription or active splicing regions.
Tissue Specificity:
Expressed in most adult human tissues, including spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Not detected in peripheral blood leukocyte. Expressed in the dermis and in all layers of the epidermis, with high levels of expression in the basal layers (at protein level). Expressed in osteoblasts (at protein level). Expressed in mesenchymal stem cells (at protein level). Expressed in breast tumor cells (at protein level).
Post-translational modifications:
GSK3B-mediated phosphorylation results in cytoplasmic localization and degradation.
DISEASE:
Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) . WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1
Similarity:
Belongs to the snail C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers.
SWISS:
O43623
Gene ID:
6591
Database links:Entrez Gene: 6591Human
Entrez Gene: 20583Mouse
Entrez Gene: 641345Pig
Entrez Gene: 25554Rat
Entrez Gene: 432368Chicken
Entrez Gene: 520631Cow
Omim: 602150Human
SwissProt: Q3MHQ4Cow
SwissProt: O43623Human
SwissProt: P97469Mouse
SwissProt: Q3UZ96Mouse
SwissProt: O08954Rat
Unigene: 360174Human
Unigene: 4272Mouse
Unigene: 43117Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
转录调节因子（Transcriptin Regulators）
锌指转录因子Slug主要用于消化系统肿瘤转移方面的研究。
- 产品图片
Sample:
Lane1: Heart (Rat) Lysate at 30 ug
Lane2: Liver (Rat) Lysate at 30 ug
Primary: Anti-SNAI2/SLUG (bs-1382R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 30kD
Observed band size : 30kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SNAI2 Polyclonal Antibody, Unconjugated(bs-1382R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining