产品中心
当前位置：首页>产品中心Anti-Glutathione Reductase
货号： bs-0837R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0837R
- 英文名称
- Glutathione Reductase
- 中文名称
- 谷胱甘肽还原酶抗体
- 别 名
- glutathione reductase; GLUR; Glutathione reductase mitochondrial; GR; Gr1; GRase; GRD 1; GRD1; GSR; MGC78522; GSHR_HUMAN.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 免疫学 激酶和磷酸酶 线粒体
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 57kDa
- 细胞定位
- 分泌型蛋白 线粒体
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Glutathione Reductase:421-522/522
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Glutathione reductase (GR) is a member of pyridine nucleotide- disulfideoxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. GR is a cytoplasmic flavoenzyme widely distributed in aerobic organisms. The dimeric protein is composed of two identical subunits, each containing 1 FAD and 1 redox-active disulfide/dithiol as components of the catalytic apparatus. It plays a role in maintaining glutathione (GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG): GSSG + NADPH + H+?2GSH + NADP+. In mosteukaryotic cells, GR maintains the ratio of [GSH]/[GSSG], and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis.
Function:
Maintains high levels of reduced glutathione in the cytosol.
Subunit:
Homodimer; disulfide-linked.
Subcellular Location:
Isoform Mitochondrial: Mitochondrion.
Isoform Cytoplasmic: Cytoplasm.
Similarity:
Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
SWISS:
P00390
Gene ID:
2936
Database links:Entrez Gene: 2936 Human
Entrez Gene: 14782 Mouse
Entrez Gene: 116686 Rat
Omim: 138300 Human
SwissProt: P00390 Human
SwissProt: P47791 Mouse
SwissProt: P70619 Rat
Unigene: 271510 Human
Unigene: 283573 Mouse
Unigene: 19721 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
胱甘肽还原酶为一种黄素酶，可催化氧化型谷胱甘肽转变成还原型谷胱甘肽(GSH)，从而提高细胞液中GSH的含量。
GSH具有抵抗自由基氧化对机体损伤的作用，GSH的重要生理功能在于能还原体内生成的过氧化氢(H2O2)。GSH的储量与创伤、肿瘤等的预后，以及人体衰老有关。
- 产品图片
Sample:
A549(Human) Cell Lysate at 30 ug
SP2/0(Mouse) Cell Lysate at 30 ug
Primary: Anti-Glutathione Reductase (bs-0837R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
Paraformaldehyde-fixed, paraffin embedded (Human Pulp); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glutathione Reductase) Polyclonal Antibody, Unconjugated (bs-0837R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.