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货号： bs-10257R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-10257R
- 英文名称
- COX4
- 中文名称
- 细胞色素c氧化酶IV亚型1抗体
- 别 名
- COX-IV; COX 4; COX 4I1; COX 4I2; COXIV Isoform 2; COX IV 1; COX IV-1; COX IV; COX4 2; COX4; COX4B; COX4I1; COX4I2; COX4L2; COXIV 2; COXIV; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit 4 isoform 1 mitochondrial; Cytochrome C Oxidase Subunit IV; Cytochrome c oxidase subunit IV isoform 1; Cytochrome c oxidase subunit IV isoform 2 (lung); dJ857M17.2; MGC72016; COX IV-1; Cox4a Cytochrome c oxidase polypeptide IV; COX41_HUMAN; COX4I1.
- Specific References (3) | bs-10257R has been referenced in 3 publications.[IF=2.10] Wang, Da-Ting, et al. "Artemisinin mimics calorie restriction to trigger mitochondrial biogenesis and compromise telomere shortening in mice." PeerJ 3 (2015): e822. WB ; Mouse.PubMed:25780774[IF=5.48] Wu, Bin, et al. "Succinate-induced neuronal mitochondrial fission and hexokinase II malfunction in ischemic stroke: Therapeutical effects of kaempferol." Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2017). WB ; Mouse.PubMed:28634116[IF=1.69] Gao, Jinhang, et al. "De-methylation of displacement loop of mitochondrial DNA is associated with increased mitochondrial copy number and nicotinamide adenine dinucleotide subunit 2 expression in colorectal cancer." Molecular medicine reports 12.5 (2015): 7033-7038. WB ; Human.PubMed:26323487
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 细胞生物 免疫学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 17kDa
- 细胞定位
- 细胞浆 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human COX4:51-169/169
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3 of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq, Jul 2008]
Function:
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Subcellular Location:
Mitochondrion inner membrane.
Post-translational modifications:
Ubiquitous.
Similarity:
Belongs to the cytochrome c oxidase IV family.
SWISS:
P13073
Gene ID:
1327
Database links:Entrez Gene: 1327 Human
Entrez Gene: 12857 Mouse
Entrez Gene: 29445 Rat
Entrez Gene: 281090 Cow
Omim: 123864 Human
SwissProt: P00423 Cow
SwissProt: Q3SZS0 Cow
SwissProt: P13073 Human
SwissProt: P19783 Mouse
SwissProt: P10888 Rat
Unigene: 433419 Human
Unigene: 386758 Mouse
Unigene: 2528 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample: kidney (Mouse) Lysate at 40 ug
Primary: Anti-COX4 (bs-10257R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 17 kD
Observed band size: 17 kD
Sample:
heart (Mouse) Lysate at 40 ug
Primary: Anti-COX4 (Bs-10257R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 17 kD
Sample: kidney (Mouse) Lysate at 40 ug
Primary: Anti-COX4 (bs-10257R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 17 kD
Observed band size: 17 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (COX4) Polyclonal Antibody, Unconjugated (bs-10257R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-COX4 Polyclonal Antibody, Unconjugated(bs-10257R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Positive control: Hela cells
Concebtration: 5μg/10^6 cells
Incubation conditions: Avoid light , 30 minutes on the ice.