产品中心
当前位置：首页>产品中心Anti-SOCS3
货号： bs-0580R 基本售价： 380.0 元 规格： 20ul
- 规格：20ul
- 价格：380.00元
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0580R
- 英文名称
- SOCS3
- 中文名称
- 细胞因子信号传导抑制蛋白3抗体
- 别 名
- suppressor of cytokine signaling 3; ATOD4; CIS 3; CIS3; Cish3; Cytokine induced SH2 protein 3; E2a Pbx1 target gene in fibroblasts 10; EF 10; MGC71791; SOCS 3; SOCS-3; SSI 3; SSI3; STAT induced STAT inhibitor 3; Suppressor of cytokine signaling 3; SOCS3_HUMAN.
- Specific References (4) | bs-0580R has been referenced in 4 publications.[IF=2.41] Liu, Wen-bin, et al. "CpG island hypermethylation of multiple tumor suppressor genes associated with loss of their protein expression during rat lung carcinogenesis induced by 3-methylcholanthrene and diethylnitrosamine." Biochemical and biophysical research communications 402.3 (2010): 507. IHC-P ; Rat.PubMed:20970405[IF=2.33] Zhu, Jian-guo, et al. "Expression of SOCSs in human prostate cancer and their association in prognosis." Molecular and cellular biochemistry (2013): 1-9. Human.PubMed:23666742[IF=1.96] Kou, Wei, et al. "Transforming growth factor-β1 promotes Treg commitment in nasal polyposis after intranasal steroid treatment." Inflammation Research (2013): 1-7. ELISA ; Human.PubMed:23178794[IF=1.55] Song, Jin-Ning, et al. "Dynamic expression of the suppressor of cytokine signaling-3 and cytokines in the cerebral basilar artery of rats with subarachnoid hemorrhage, and the effect of acetylcholine." Acta Neurochirurgica (2014): 1-9. IHC-P ; Rat.PubMed:24463741
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 细胞生物 免疫学 神经生物学 信号转导
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit,
- 产品应用
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 25kDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Socs 3:155-225/225
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Cytokines signal transduction pathway suppressor of cytokine signaling 3 positive cells in the brain are widely distributed, some of the cytoplasm of neurons, nerve fibers, glial cell line. suppressor of cytokine signaling 3 studies in recent years to prove: vascular endothelial cells also showed immune SOCS 3-positive.
Function:
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST (By similarity).
Subunit:
Interacts with multiple activated proteins of the tyrosine kinase signaling pathway including IGF1 receptor, insulin receptor and JAK2. Binding to JAK2 is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK2 JH1 domain. Binds specific activated tyrosine residues of the leptin, EPO, IL12, GSCF and gp130 receptors. Interaction with CSNK1E stabilizes SOCS3 protein. Component of the probable ECS(SOCS3) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SOCS3. Interacts with CUL5, RNF7, TCEB1 and TCEB2. Interacts with CUL2.
Tissue Specificity:
Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
Post-translational modifications:
Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1.
DISEASE:
Genetic variation in the promoter region of SOCS3 may be associated with susceptibility to atopic dermatitis 4 (ATOD4) [MIM:605805]. Atopic dermatitis [MIM:603165], also known as eczema commonly begins in infancy or early childhook and is characterized by ichy and inflamed skin.
Similarity:
Contains 1 SH2 domain.
Contains 1 SOCS box domain.
SWISS:
O14543
Gene ID:
9021
Database links:Entrez Gene: 9021 Human
Entrez Gene: 89829 Rat
Omim: 604176 Human
SwissProt: O14543 Human
SwissProt: O88583 Rat
Unigene: 527973 Human
Unigene: 127801 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
细胞因子信号转导途径抑制蛋白SOCS3阳性细胞在脑内分布广泛,阳性产物主要神经元核内;部分神经元的胞浆、神经纤维、胶质细胞。
细胞激酶信号-3抑制剂近年研究证明：血管内皮细胞也呈SOCS 3免疫阳性.
- 产品图片
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SOCS3 Polyclonal Antibody, Unconjugated(bs-0580R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SOCS3 Polyclonal Antibody, Unconjugated(bs-0580R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining