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货号： bs-0687R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0687R
- 英文名称
- OAS1
- 中文名称
- 寡腺苷酸合成酶-1
- 别 名
- 2,5-oligoadenylate synthetase 1; (2 5)oligo(A) synthetase 1 ; (2 5)oligo(A) synthetase 1; 2 5 Oligoadenylate Synthetase 1; 2 5A synthetase 1; 2 5 oligo A synthetase 1; 2 5 oligoadenylate synthetase 1; 2 5 oligoisoadenylate synthetase 1; 25 oligo A synthetase 1; 25 oligoadenylate synthetase 1; 25 oligoisoadenylate synthetase 1; E18/E16; IFI 4; IFI4; OAS 1; OIAS; OIASI; p46/p42 OAS; OAS1_HUMAN.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 激酶和磷酸酶
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Pig,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 40/46kDa
- 细胞定位
- 细胞核 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human OAS1:1-100/400
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This protein is an enzyme included in the 2, 5 oligoadenylate synthase family. This enzyme is induced by interferons and catalyzes the 2, 5 oligomers of adenosine in order to bind and activate RNase L. This enzyme family plays a significant role in the inhibition of cellular protein synthesis and viral infection resistance.
Function:
Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2-5-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. The secreted form displays antiviral effect against vesicular stomatitis virus (VSV), herpes simplex virus type 2 (HSV-2), and encephalomyocarditis virus (EMCV) and stimulates the alternative antiviral pathway independent of RNase L.
Subunit:
Monomer. Homotetramer.
Subcellular Location:
Cytoplasm. Mitochondrion. Nucleus. Microsome. Endoplasmic reticulum. Secreted. Note=Associated with different subcellular fractions such as mitochondrial, nuclear, and rough/smooth microsomal fractions.
Similarity:
Belongs to the 2-5A synthase family.
SWISS:
P00973
Gene ID:
4938
Database links:Entrez Gene: 347699 Cow
Entrez Gene: 4938 Human
Entrez Gene: 397570 Pig
Entrez Gene: 192281 Rat
Omim: 164350 Human
SwissProt: P00973 Human
SwissProt: P11928 Mouse
SwissProt: Q29599 Pig
SwissProt: Q05961 Rat
Unigene: 524760 Human
Unigene: 10383 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
用于免疫调控通路的研究。主要用于系统性红斑狼疮方面的研究。
- 产品图片
Sample:
Hela(Human) Cell Lysate at 40 ug
Primary: Anti-OAS1 (bs-0687R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40/46 kD
Observed band size: 40 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-OAS1 Polyclonal Antibody, Unconjugated(bs-0687R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining