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货号： bs-0342R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0342R
- 英文名称
- Integrin beta 3
- 中文名称
- 整合素β3抗体
- 别 名
- Integrin beta-3; Integrin beta chain, β3 precursor; Integrin Beta 3; CD 61; CD61; CD61 antigen; GP3A; GPIIIa; HPA 1; HPA 4; Integrin beta 3 (platelet glycoprotein IIIa antigen CD61); Integrin beta chain beta 3; ITG B3; ITGB 3; ITGB3; NAIT; Platelet fibrinogen receptor beta subunit; Platelet glycoprotein IIIa; platelet glycoprotein IIIa precursor; Platelet membrane glycoprotein IIIa; PTP; ITB3_HUMAN. Integrin β3; Integrin-β3; Integrin β 3; Integrinβ3;
- Specific References (5) | bs-0342R has been referenced in 5 publications.[IF=7.60] Wang, Yang, et al. "Induced apoptosis of osteoblasts proliferating on polyhydroxyalkanoates." Biomaterials (2013). ELISA ; Rat.PubMed:23433672[IF=2.97] Zhou, Jianhong, et al. "Sophoricoside fails the embryo implantation by compromising the uterine endometrial receptivity at implantation window of pregnant mice." Chemico-Biological Interactions (2014). WB ; Mouse.PubMed:24877640[IF=3.19] Zhang, Lei, et al. "High-Throughput RNAi Screening Identifies a Role for the Osteopontin Pathway in Proliferation and Migration of Human Aortic Smooth Muscle Cells." Cardiovascular Drugs and Therapy (2016): 1-15. IF(ICC) ; Human.PubMed:27095116[IF=1.40] Li, Feng, et al. "Elevated expression of integrin αv and β5 subunit in laryngeal squamous-cell carcinoma associated with lymphatic metastasis and angiogenesis." Pathology-Research and Practice 209.2 (2013): 105-109. IHC-P ; Human.PubMed:23261238[IF=2.44] Qu, Xin-Lan, et al. "Effect of 2, 3′, 4, 4′, 5-Pentachlorobiphenyl Exposure on Endometrial Receptivity and the Methylation of HOXA10." Reproductive Sciences (2017): 1933719117711258. IHC-P ; Mouse.PubMed:28631552
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 细胞生物 免疫学 信号转导 干细胞 细胞粘附分子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Pig, Cow, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 84kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Integrin beta 3:27-120/788 <Extracellular>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The ITGB3 (Integrin beta chain beta 3) protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
Function:
Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposis sarcoma lesions.
Subunit:
Heterodimer of an alpha and a beta subunit. Beta-3 associates with either alpha-IIb or alpha-V. Isoform Beta-3C interacts with FLNB. Interacts with COMP. Interacts with HIV-1 Tat. Interacts with PDIA6 following platelet stimulation. Interacts with SYK; upon activation by ITGB3 promotes platelet adhesion. Interacts with MYO10.
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Tissue Specificity:
Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
Post-translational modifications:
Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.
DISEASE:
Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT variants have normal or near normal (60-100%) expression of dysfunctional receptors.
Similarity:
Belongs to the integrin beta chain family. Contains 1 VWFA domain.
SWISS:
P05106
Gene ID:
3690
Database links:Entrez Gene: 3690 Human
Entrez Gene: 16416 Mouse
Entrez Gene: 29302 Rat
Omim: 173470 Human
SwissProt: P05106 Human
SwissProt: O54890 Mouse
Unigene: 218040 Human
Unigene: 87150 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
CD61抗原又称为GP III a，是一种表达于血小板、巨核细胞、单核细胞、巨噬细胞和内皮细胞上的糖蛋白。CD61和CD41构成血小板糖蛋白II b/III b。
- 产品图片
Tissue/cell: human rectal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Integrin beta 3/CD61 Polyclonal Antibody, Unconjugated(bs-0342R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Overlay histogram showing HL 60 cells stained with bs-0342R (Green line).
The cells were fixed with 90% methanol (5 min) and then permeabilized with 0.01M PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (bs-0342R,1μg/1x10^6 cells) for 30 min at 22℃. The secondary antibody used was fluorescein isothiocyanate goat anti-rabbit IgG (H+L) (bs- 0295G-FITC , Brillant blue line) at 1/200 dilution for 30 min at 22℃. Isotype control antibody was rabbit IgG (polyclonal,bs-0295P,Orange line) (1μg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of 20,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.