p27[Kip1] Pure 57/Kip1/p27 150ug
货号：
610242 基本售价：
7885.0 元 规格：
150ug
产品信息
概述| 货号 | 610242 |
| 描述 | Kip1 is a cyclin-dependent kinase (cdk) inhibitor that was identified as a result of its role in TGF β -induced G1 phase arrest and cell-cell contact. In vitro , p27 [Kip1] binds tightly to Cyclin D-Cdk4, Cyclin E-Cdk2, and Cyclin A-Cdk2 complexes and inhibits their activity. In normal cells, Kip1 is sequestered and its activity gradually decreases as cells reach S phase. The addition of TGF- β in early G1 blocks this decline in activity, by preventing the sequestration of Kip1. TGF- β also reduces the levels of Cdk4. Kip1 preferentially binds Cyclin D-Cdk4, but the lower levels of Cdk4 in TGF- β treated cells allow Kip1 to be available for binding to Cyclin E-Cdk2 and Cyclin A-Cdk2. Kip1 is structurally related to Cip1/WAF1, having a similar 60 amino acid sequence in the N-terminal region. A 52 amino acid Kip1 peptide (residues 28-79) from this region is sufficient to inhibit cdk activity in vitro. |
| 同种亚型 | IgG1 |
| 反应种属 | Human (QC Testing) Mouse, Rat, Dog, Chicken, Frog (Tested in Development) |
| 来源宿主 | Mouse |
| 使用方法 | WB, IHC, IP, IF |
性能| 供应商 | BD Pharmingen |
| 免疫原 | Mouse Kip1 aa. 1-197 |
| 存放说明 | Store undiluted at -20°C.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
|
| 存储溶液 | Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide. |
| 浓度 | 250 µg/ml |
