Staining of rat spleen cells with Mouse anti H-2I-Ak/s (MCA2687GA)

Staining of rat spleen cells with Mouse anti H-2I-Ak/s: Alexa Fluor^® 647 (MCA2687A647)

Staining of rat spleen cells with Mouse anti H-2I-Ak/s: Alexa Fluor^® 488 (MCA2687A488)

Staining of rat spleen cells with Mouse anti H-2I-Ak/s: FITC (MCA2687FA)

Staining of rat spleen cells with Mouse anti H-2I-Ak/s: FITC (MCA2687FB)

Staining of rat spleen cells with Mouse anti H-2I-Ak/s: RPE (MCA2687PE)

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Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used for the identification of MHC class II expressing rat retinal microglia by immunofluorescence on cryostat sections.
Image caption:
Activation of microglial cells. Vertical section of retinas from a SD (A-C), untreated P23H (D-F) and tauroursodeoxycholic acid (TUDCA)-treated P23H (G-I) rat stained for Iba1 (green; A, D, G) MHC-II RT 1B (red; B, E, H) or both (C, F, I). Nuclei stained with a nuclear marker (TO-PRO 3, blue). All images were collected from the central area of the retina, close to the optic nerve. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer. Scale bar: 20 μm.

From: Noailles A, Fernández-Sánchez L, Lax P, Cuenca N. Microglia activation in a model of retinal degeneration and TUDCA neuroprotective effects.
J Neuroinflammation. 2014 Oct 29;11(1):186.

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Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used for the identification of MHC class II expressing mictoglial cells in rat brain by immunohistochemistry on gormalin fixed cryosections.
Image caption:

Visualization of microglia in midbrain with GSA-I-B4 lectin (A-F) and in motor cortex with OX-42 (G) and OX-6 (H) during symptomatic disease. A, low power view of midbrain reveals enhanced lectin staining in the red nucleus. B, higher magnification shows that enhanced lectin reactivity is confined strictly to red nucleus region (arrows indicate perimeter of red nucleus). C, microglial fusions are interspersed with rubrospinal neurons that appear undamaged. D, lectin-positive microglial fusion (giant cell) within red nucleus. E, oculomotor nucleus reveals normal-appearing motor neurons and lack of microgliosis. F, substantia nigra (pars compacta) shows presence of normal, ramified microglial cells. G, motor cortex shows normal, ramified microglia. H, single, ramified microglial cell positive with OX-6 (arrow) near lateral ventricle. Scale bars: 400 μm (A); 200 μm (E); 100 μm (B,H); 50 μm (C,F,G); 20 μm (D).

From: Fendrick SE, Xue QS, Streit WJ. Formation of multinucleated giant cells and microglial degeneration in rats expressing a mutant Cu/Zn superoxide dismutase gene.
J Neuroinflammation. 2007 Feb 28;4:9.

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Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used for the identification of MHC class II expressing cells in the rat brain by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative images of (immuno)histochemical staining of brain tissue (TBI and control) with ED-1, OX-6, GFAP, Perl's, and Fluoro-Jade B ten days after the microdialysis experiment.

From: Folkersma H, Foster Dingley JC, van Berckel BN, Rozemuller A, Boellaard R, Huisman MC, Lammertsma AA, Vandertop WP, Molthoff CF. Increased cerebral (R)-[(11)C]PK11195 uptake and glutamate release in a rat model of traumatic brain injury: a longitudinal pilot study. J Neuroinflammation. 2011 Jun 14;8:67.

Published customer image:Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used for the visualization of MHC Class II expressing cells in Rat brain by immunohistochemistry on formalin fixed coronal tissue sections.
Image caption:
Progressive degeneration of the nigral dopaminergic neurons after intrastriatal LPS. (A) Representative TH immunostaining of coronal midbrain sections demonstrates that the numbers of TH-positive neurons and fibers in the substantia nigra pars compacta are gradually reduced by intrastriatal LPS injection. Note that TH-positive neurons in the medial substantia nigra pars compacta and ventral tegmental area are spared; scale bar: 200 μm. (B) Stereological cell counts of the TH-positive neurons in the substantia nigra pars compacta (n = 5–6/group, ** p<0.01, *** p<0.001). (C) The substantia nigra pars compacta is outlined with an orange dashed line (top). High magnification image of Nissl stainings suggest loss of the nigral dopaminergic neurons, at four weeks following LPS injection (bottom); scale bar: 200 μm. (D) Silver staining is hardly seen in the substantia nigra ipsilateral to vehicle treatment. However, abundant silver grain-deposits are observed in the neurons (arrows) and fibers (arrow heads) in the substantia nigra ipsilateral to the intrastriatal LPS injections, indicating there is ongoing neurodegenerative process in the region. Scale bar: 20 μm.

From: Choi D-Y, Liu M, Hunter RL, Cass WA, Pandya JD, et al. (2009) Striatal Neuroinflammation Promotes Parkinsonism in Rats.
PLoS ONE 4(5): e5482.

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Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used for the evaluation of MHHC class II reactivity in rat brain be immunohistochemistry on coronal sections.
Image caption:
OX-6 IHC staining in the DG. No OX-6 positive cells were identified in P1 D1D2 pups treated with either NS (a) or Dex (b). Brain tumor implant staining as a positive control showed OX-6 positive cells stained brown (c), arrow). Magnification, 400x; scale bar, 50μm.

From: Sze CI, Lin YC, Lin YJ, Hsieh TH, Kuo YM, Lin CH. The role of glucocorticoid receptors in dexamethasone-induced apoptosis of neuroprogenitor cells in the hippocampus of rat pups.
Mediators Inflamm. 2013;2013:628094.

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Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used for the evaluation of MHC class II expression in the rat brain of transgenic rats by immunohistochemistry on cryostat sections.
Image caption:
Expression of MHC class II molecules is different in brains of transgenic rat models. In brainstem of WKY TG rats (A), activation of microglia is accompanied by widespread MHCII expression, while in SHR TG (B), only sparse MHCII staining was recorded. Stereological quantification shows highly significant differences between the transgenic lines. In WKY TG rats, there are 10 times more microglia that express MHCII than are present in SHR TG rats (C, Student's t-test, *** p < 0.001). Pre-fixed frozen sections. Scale bars: 50 μm.

From: Stozicka Z, Zilka N, Novak P, Kovacech B, Bugos O, Novak M. Genetic background modifies neurodegeneration and neuroinflammation driven by misfolded human tau protein in rat model of tauopathy: implication for immunomodulatory approach to Alzheimer's disease.
J Neuroinflammation. 2010 Oct 12;7:64.

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Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX6 used for the identification of activated microglia in rat brain be immunohistochemistry on formalin fixed cryosections.
Image caption:
Neuroprotection against 6-OHDA toxicity following delayed subchronic intranigral administration of 2R,4R-APDC is associated with reduced numbers of activated microglia in the SNc. Representative photomicrographs (40) of DAB/peroxidase staining for OX-6, a marker of activated microglia in the SNc from each treatment group (scale bar 200μm.). (a) 6-OHDA + vehicle, unlesioned SNc, (b) 6-OHDA + vehicle, lesioned SNc, (c) 6-OHDA + 2R,4R-APDC (10?nmol) unlesioned SNc, (d) 6-OHDA + 2R,4R-APDC (10?nmol) lesioned SNc. Note the increase in intensity of OX-6 staining and the increase in OX-6+ cells displaying morphology of activated microglia in the lesioned SNc of vehicle-treated animal, which appears markedly reduced in animals treated with 2R,4R-APDC.

From: Chan H, Paur H, Vernon AC, Zabarsky V, Datla KP, Croucher MJ, Dexter DT. Neuroprotection and Functional Recovery Associated with Decreased Microglial Activation Following Selective Activation of mGluR2/3 Receptors in a Rodent Model of Parkinson's Disease.
Parkinsons Dis. 2010 May 23;2010. pii: 190450.

Puiblished customer image:
Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used to identify activated microglia in rat brain be immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Imatinib reduces infiltration of immune cells and attenuates microglia activation. IHC analysis on paraffin embedded spinal cord tissue cross-sections on day 10 p.i. (A–D; n = 8 rats/experimental group; representative images shown) and 14 p.i. (E–H; n = 5 rats/experimental group; representative images shown). Although showing no sign of CNS inflammation and demyelination, control animals already started recruiting W3/13+ T-cells (B) and ED1+ macrophages (D) to the meninges and in the perivascular space, whereas the imatinib-treated group showed a delay in recruitment of inflammatory cells to the CNS. On day 14 p.i., spinal cords of the imatinib-treated rats exhibiting demyelinated lesions recruited lower amounts of W3/13+ T-cells (G, H) while ED1+ macrophages infiltration was similar to the controls (E, F). Scale bar, 200 μm (A–H). (I–Z) IF performed on spinal cord cross-sections of rats injected with fluorescent tracer (dextran, red) on day 14 p.i. α-Ox-42, ED1, Ox-6, Ox-22, CD45RA and W3/13 antibody staining (all in green) in imatinib- (I, L, O, R, U, X) and PBS-treated rats (J, M, P, S, V, Y). (I–K) Microglia activation was significantly decreased in the imatinib-treated rats, while Ox-42+ cells were detectable around leaky blood vessels (asterix) in the control tissue. (L–N) The amount of macrophages/activated microglia cells was significantly decreased in the spinal cords of the imatinib-treated rats. (O–Q) Significantly lower amounts of MHC class II+ cells were found in the meninges and parenchyma of the imatinib-treated rats vs. PBS controls. (R–Z) Significantly lower amounts of Ox-22+, CD45RA+ and W3/13+ cells were found in the meninges and parenchyma of the imatinib-treated rats vs. PBS controls (R, U, X vs. S, V, Y). Quantifications of Ox-42, ED1, Ox-6, Ox-22, CD45RA and W3/13 expression based on green fluorescent pixel area quantifications from spinal cord cross-sections (K, N, Q, T, W, Z). n = 5 rats/experimental group. Scale bar, 50 μm. Error bars, S.E.M. Statistics were calculated using t-test and P values <0.05 were considered significant (P<0.05 = *, P<0.01 = **, P<0.001 = ***). Imatinib or PBS oral gavage was performed from day 5 p.i until the end of the experiment.

From: Z. Adzemovic M, Zeitelhofer M, Eriksson U, Olsson T, Nilsson I (2013) Imatinib Ameliorates Neuroinflammation in a Rat Model of Multiple Sclerosis by Enhancing Blood-Brain Barrier Integrity and by Modulating the Peripheral Immune Response.
PLoS ONE 8(2): e56586.

Published customer image:Mouse anti MHC Class II H-2I-Ak/s antibody, clone OX-6 used to identify activated microglia in the rat hippocampus by immunohistochemistry on formalin fixed cryosections.
Image caption:
Microglial activation in response to the “Binge Drinking” regime and β-sultam supplementation. Representative microphotographs of OX-6 positive microglia in the hippocampus of binge-treated rats without (upper panels) and with β-sultam supplementation (lower panels). The micrograph shows colocalisation of OX-6 and iNOS immunoreactivities. Black arrows indicate iNOS, red arrows indicate activated microglia. b: Quantitation of microglial activation is presented. Number of OX-6 immunopositive cells (3-4 rats per group) analysed by two way ANOVA, where the two factors were Binge regime and β-sultam supplementation, followed by the post hoc Fisher's LSD (Protected t-Test) for multiple comparisons. Binge F2,14=66.24, p<0.0001; ethane-β- sultam: F1,14=14.50, p<0.0019; Interaction binge x ethane-β-sultam: F2,14=6.12, p<0.0123; post hoc Bonferroni multiple comparison test: *p<0.05, **p<0.01, ***p<0.001 versus control, #p<0.5, ##<<0.01, ###p<0.001 versus Binge alone.

From: Stefanini C, Colivicchi MA, Della Corte L, Ward RJ, de Witte P, et al. (2014) Ethane-β-Sultam Modifies the Activation of the Innate Immune System Induced by Intermittent Ethanol Administration in Female Adolescent Rats. J Alcohol Drug Depend 2:150.