NF-Kpa-B BV421 K10-895.12.50 50Tst
货号：
565446 基本售价：
5830.0 元 规格：
50Tst
产品信息
概述| 货号 | 565446 |
| 描述 | The K10-895.12.50 monoclonal antibody recognizes the phosphorylated serine 529 (pS529) in the transactivation domain of the human NF- κ B p65 subunit. Nuclear factor κ B (NF- κ B) is a ubiquitously expressed transcription factor that regulates the expression of many other genes. It is crucial for cellular responses to a variety of stimuli including stress and microbial pathogens that lead to immunity, inflammation, proliferation, differentiation, survival, apoptosis, and tumorigenesis. The most studied NF- κ B complex consists of the p50 (also known as NF- κ B1) and p65 (also known as REL-A) subunits, both containing a 300-amino acid region with homology to the Rel proto-oncogene product (RH domain). The RH domain contains motifs for dimerization, nuclear localization, and binding to specific DNA sequences. In addition to the RH domain, the p65 subunit contains the transactivation domain, which is responsible for the interaction with the inhibitor I κ B and which contains phosphorylation sites. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to I κ B. This complex prevents nuclear translocation and activity of NF- κ B. In response to stimuli such as cytokines, LPS, DNA damage, and microbial infections, I κ B is phosphorylated at critical residues. This phosphorylation induces dissociation of the I κB/NF-κ B complex, allowing the free heterodimeric NF- κ B to translocate to the nucleus. Furthermore, optimal activation of NF- κ B requires phosphorylation in the transactivation domain of p65. In the nucleus, activated NF- κ B dimers bind to the κ B sites within promoters and enhancers and function as transcriptional regulators. |
| 别名 | RELA; REL-A; NFKBp65; NFKB3; Transcription factor p65; TF65 |
| 同种亚型 | Mouse BALB/c IgG2b, κ |
| 反应种属 | Human (QC Testing) |
性能| 供应商 | BD Pharmingen |
| 免疫原 | Phosphorylated Human NF-κB p65 Peptide |
| 标记 | BV421 |
| 存放说明 | Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.The antibody was conjugated with BD Horizon™ BV421 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV421 were removed.
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| 存储溶液 | Aqueous buffered solution containing BSA and ≤0.09% sodium azide. |
