| 货号 | HCA177P |
| 克隆号 | AbD18018_hIgG1 |
| 同种亚型 | IgG1 |
| 来源宿主 | HuCAL |
| 应用 | E |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at -70oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Human anti Trastuzumab (Herceptin® specificity ELISA (clone AbD18018 in IgG1 format) A microtiter plate was coated over night using various antigens (A: 20% human serum, B: IgG1/kappa from human myeloma plasma, C: Rituximab, D: Bevacizumab, E: Ustekinumab, F: Infliximab, G: Adalimumab, H: Trastuzumab as coating components. After washing and bolcking with PBST+5% BSA, detection was perfomed using Human anti Trastuzumab:HRP in its IgG1 format (clone AbD18018_hIgG1, HCA177) as the detection reagent | |
Human anti trastuzumab (Herceptin®) bridging ELISA for Anti-Drug Antibody (ADA) assay development (clone AbD18018 in IgG1 format) A microtitre plate was coated over night with trastuzumab at a concentration of 0.5 μg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of Human anti Trastuzumab, clone AbD18018_hIgG1 (HCA177) and HRP conjugated Trastuzumb as the detection reagent | |
Trastuzumab (Herceptin®) bridging ELISA for Pharmacokinetic (PK) assay development (clone AbD18018 in IgG1 format) A microtiter plate was coated over night with Human anti trastuzumab, clone AbD16712 (HCA166) (black) or clone AbD18141 (HCA169) (red) at a concentration of 1 μg/ml. After washing and blocking with PBST +5% BSA in, 10 % human serum was added spiked with increasing concentrations of trastuzumab. Detection was performed by HRP-conjugated Human anti trastuzumab, AbD16712-hIgG1 (HCA177P) at a concentration of 2 μg/ml in HiSpec assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate |
