SV40 Large T Antigen Pure PAb101 100ug
货号：
554149 基本售价：
5560.0 元 规格：
100ug
产品信息
概述| 货号 | 554149 |
| 描述 | Simian virus 40 is a small DNA virus encoded by 5.2 kb of double-stranded DNA. SV40 large T antigen (T-ag) is a multifunctional ~85 kD phosphoprotein, which is the sole viral protein required for SV40 replication. All other factors are provided by the infected host cell. In addition to its role in SV40 DNA replication, T-ag also causes transformation of susceptible cell lines. Studies of various mutant T-ag proteins have shown that the replication and transformation fractions of T-ag can be separated. The multifunctional nature of this protein has resulted in its use as a model system in a wide variety of disciplines. SV40 T-ag exercises negative regulation on the transcription of SV40 early mRNA by feedback inhibition and exerts positive regulation on transcription from the late promoter. In addition to transcriptional regulation, T-ag is involved in viral DNA replication. Specific biochemical functions required for DNA synthesis that are inherent to the T-ag include high-affinity binding to sites within the viral origin of DNA synthesis, and ATPase and helicase activities. Other functions attributed to T-ag include cellular transformation, induction of cellular DNA synthesis, induction of rRNA synthesis and provision of a host-range function for viral replication. However, all functions of T-ag are influenced by a wide range of post-translational modifications including phosphorylation, glycosylation, acetylation, acylation and adenylation. T-ag exists in monomeric as well as polymeric forms and associates with the tumor suppressor proteins p53 and Rb (retinoblastoma protein). Most of T-ag is transported to the nucleus, while a small fraction is localized at the cell surface. PAb 101 recognizes a C-terminal epitope within the last 190 amino acids of T-ag. PAb 101 was originally known as Clone 7. Studies have suggested that PAb 101 binds the strongest to mature T-ag. PAb 101 (i.e., Clone 7) was developed along with a panel of monoclonal antibodies where SV40-transformed BALB/c mouse cell lines (SVT2 or B4) were used as immunogens. The specificity of the antibody was originally characterized by a variety of techniques using SV40-infected and SV40-transformed cells. |
| 同种亚型 | IgG2a |
| 反应种属 | Viral (QC Testing) |
| 来源宿主 | Mouse |
| 使用方法 | WB, IHC(F), IP, IF |
性能| 供应商 | BD Pharmingen |
| 免疫原 | SV40-transformed BALB/c mouse cell lines |
| 存放说明 | The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at 4°C.
|
| 存储溶液 | Aqueous buffered solution containing ≤0.09% sodium azide. |
| 浓度 | 0.5 mg/ml |
