| 货号 | MCA1396 |
| 克隆号 | AD1.1.10 |
| 同种亚型 | IgG1 |
| 反应种属 | Synthetic Peptide |
| 来源宿主 | Mouse |
| 应用 | E, IP, WB |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag (MCA1396) | |
Human anti Human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualised with Mouse anti-HIS tag: FITC antibody (MCA1396F) | |
Western blot of HIS tagged protein probed with Mouse anti Histidine tag : Biotin (MCA1396B) and visualised with Streptavidin : HRP (STAR5B) | |
Western blot of HIS tagged protein probed with Mouse anti Histidine tag:Dylight®800 (MCA1396D800GA). Visualised using a LI-COR Odyssey® infrared imaging system | |
Western blot of HIS-tagged protein probed with Mouse anti Histidine tag (MCA1396G) visualised with Rabbit anti Mouse IgG : HRP (STAR13B) | |
Published customer image: Horseradish peroxidase conjugated Mouse anti Histidine tag antibody clone AD1.1.0 (MCA1396P) used for the detection of recombinant scTCR varients by western blotting. Image caption: 4B2A1 scTCR and rFkpA expression level. Western blot showing periplasmic expression levels of 4B2A1 scTCR variants and rFkpA from pHOG, pFKPEN (pEN) and pFKPEI (pEI) vectors under different expression conditions, detected with anti-His tag -HRP Ab and anti-Flag- M2 HRP Ab as described. The scTCR and rFkpA were developed with SuperSignal West Pico substrate and exposed to BioMax MR film for for 30 min and 30 sec, respectively, for the upper and lower panels. The expression analysis was repeated twice, and representative results are shown. From: Gunnarsen KS, Lunde E, Kristiansen PE, Bogen B, Sandlie I, Løset GÅ. Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA. BMC Biotechnol. 2010 Feb 3;10:8. | |
Published customer image: Horseradish peroxidase conjugated Mouse anti Histidine tag antibody clone AD1.1.0 (MCA1396P) used for the detection of recombinant scTCR varients by western blotting. Image caption: 7A10B2 scTCR and rFkpA expression level. Western blot showing periplasmic expression levels of 7A10B2 scTCR variants and rFkpA from pHOG, pFKPEN (pEN) and pFKPEI (pEI) vectors under different expression conditions, detected with anti-His tag-HRP and anti-Flag-M2-HRP as described. The scTCR and rFkpA were developed with SuperSignal West Pico substrate and exposed to BioMax MR film for for 30 min and 30 sec, respectively, for the upper and lower panels. The expression analysis was repeated twice, and representative results are shown. From: Gunnarsen KS, Lunde E, Kristiansen PE, Bogen B, Sandlie I, Løset GÅ. Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA. BMC Biotechnol. 2010 Feb 3;10:8. | |
Published customer image: Horseradish peroxidase conjugated Mouse anti Histidine tag antibody clone AD1.1.0 (MCA1396P) used for the detection of recombinant scTCR varients by western blotting. Image caption: Purity of proteins. (A) and (B); S75 size exclusion chromatography of scTCR 4B2A1 Vαβ and Vβα respectively. 50 μl of the IMAC purified material was subject to the purification procedure. In (A) fractions A14-B1 corresponds to the higher aggregates, while fraction B5-B7 corresponds to the monomeric scTCR 4B2A1 Vαβ, the monomeric fraction is highlighted with an arrow. In (B) fraction A15-B1 and B3-B4 corresponds to the higher aggregates, while fraction B6-B7 corresponds to the monomeric scTCR 4B2A1 Vβα, the monomeric fraction is highlighted with an arrow. (C) and (D); Western blots showing reduced and non-reduced scTCR Vαβ and Vβα after IMAC purification and S75 size exclusion chromatography. Samples of 0.25 μg were loaded on the gel and analysed with anti-His tag-HRP. From: Gunnarsen KS, Lunde E, Kristiansen PE, Bogen B, Sandlie I, Løset GÅ. Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA. BMC Biotechnol. 2010 Feb 3;10:8. |
