DNA-PK Pure 4F10C5 100ug
货号：
556456 基本售价：
5360.0 元 规格：
100ug
产品信息
概述| 货号 | 556456 |
| 描述 | The ability of mammalian cells to detect and repair DNA damage is essential in maintaining the structural integrity of their genes. Cells have evolved a number of repair pathways in order to repair different types of DNA lesions. DNA-dependent protein kinase (DNA-PK) is a serine-threonine protein kinase that is involved in DNA double-stranded break repair and variable-diversity-joining [V(D)J] recombination. To be active, DNA-PK must bind to double-stranded DNA containing broken ends, nicks, or single stranded gaps. Activated DNA-PK consists of three polypeptide components: Ku-80, Ku-70, and an ~350 kDa catalytic subunit (p350). First, Ku-80 and Ku-70 associate with each other to form a heterodimeric complex that binds to DNA breaks. By its interaction with DNA, Ku is thought to target p350 to sites of DNA damage. p350 then binds to the Ku-70/80 heterodimer (Ku) resulting in production of the active DNA-PK. Active DNA-PK phosphorylates a number of substrates in vitro, including proteins involved in the response of cells to DNA damage (p53, Ku, and replication protein A), and transcription factors (Sp1, Oct 1, Oct 2, SRF, Fos, and Jun). Clone 4F10C5 has been reported to recognize the 350 kDa catalytic subunit of DNA-PK. |
| 别名 | DNA Dependent Protein Kinase |
| 同种亚型 | IgG1 |
| 反应种属 | Human (QC Testing) |
| 来源宿主 | Mouse |
| 使用方法 | WB, IP |
性能| 供应商 | BD Pharmingen |
| 免疫原 | Human DNA-PK (p350) aa. 277-531 Recombinant Protein |
| 存放说明 | Store undiluted at 4°C.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
|
| 存储溶液 | Aqueous buffered solution containing ≤0.09% sodium azide. |
| 浓度 | 0.5 mg/ml |
