| 货号 | HCA216 |
| 克隆号 | AbD19376_hIgG1 |
| 同种亚型 | IgG1 |
| 来源宿主 | HuCAL |
| 应用 | E |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at -70oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. |
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HCA216 Specificity ELISA To confirm antibody specificity, a microtiter plate was coated overnight with various antigens at a concentration of 5µg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP conjugated HCA216 at 2µg/ml, in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate | |
Infliximab binds to HCA216 in the presence of TNF-α To assess the ability of HCA216 to inhibit the binding of Infliximab to its target molecule, a microtiter plate was coated overnight with HCA216 (as Fab fragment) at 5.0 µg/mL. After washing and blocking with PBST+5% BSA, Infliximab alone (red circles), or with 5.0 fold molar excess of TNF-α (black squares) was added in the given concentrations. Free or TNF-α bound Infliximab binding to HCA216 was detected using Mouse anti Human IgG (Fc) CH2 domain:HRP (MCA647P) | |
Infliximab Bridging ELISA for Pharmacokinetic (PK) Assay Development A microtiter plate was coated overnight with HCA214 at a concentration of 1µg/ml. After washing and blocking with PBST+5% BSA, Infliximab spiked in 10% human serum was added at the given concentrations. Detection was performed using HRP-conjugated HCA216 at 2µg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate | |
Infliximab Bridging ELISA for Pharmacokinetic (PK) Assay Development A microtiter plate was coated overnight with HCA212 at a concentration of 5µg/ml. After washing and blocking with PBST+5% BSA, Infliximab spiked in 10% human serum was added at the given concentrations. Detection was performed using HRP-conjugated HCA216 at 2µg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate | |
HCA216 Bridging ELISA for Anti-Drug Antibody (ADA) Assay Development A microtiter plate was coated overnight with Infliximab at a concentration of 1µg/ml. After washing and blocking with PBST+5% BSA, anti Infliximab HCA216 spiked in 10% human serum was added at the given concentrations. Detection was performed using HRP-conjugated Infliximab |
