| 货号 | HCA228 |
| 克隆号 | AbD19376_hIgG1 |
| 同种亚型 | IgG1 |
| 来源宿主 | HuCAL |
| 应用 | E |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at -70oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. |
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Human anti cetuximab specificity ELISA A microtiter plate was coated overnight with various antigens at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP conjugated Human anti cetuximab (clone AbD19376_hIgG1) at a concentration of 2 µg/ml and QuantaBlu™ fluorogenic peroxidase substrate | |
Human anti cetuximab specificity titration ELISA A microtiter plate was coated over night with human IgG1/kappa, human IgG1/lambda, rituximab or cetuximab at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, detection was performed using Human anti cetuximab (clone AbD19376_hIgG1 in a monovalent format) titrated to the given concentrations in PBST+10% human serum, followed by Mouse anti histidine tag:HRP (MCA5995P) in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate | |
Cetuximab bridging ELISA for Pharmacokinetic (PK) assay development A microtiter plate was coated over night with Human anti cetuximab (clone AbD19834, HCA220) at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of cetuximab. Detection was performed using HRP-conjugated Human anti cetuximab (clone AbD19376_hIgG1) at a concentration of 2 µg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate | |
Cetuximab inhibition ELISA A microtiter plate was coated over night with EGFR at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, a pre-incubated mixture of cetuximab (CTX, 0.3 µg/ml) plus increasing concentrations of the non-inhibitory Human anti cetuximab (HCA228) was added. In parallel experiments, HCA228 was replaced with inhibitory Human anti cetuximab antibodies of varying affinity (HCA220,HCA221orHCA223). For this assay, HCA221, HCA223 and HCA228 were used in their monovalent Fab format. Free cetuximab, still capable of binding to the EGFR-coated plate, was detected using Goat anti Human IgG F(ab)2:HRP (STAR126P) |
