Cytochrome c Pure 7H8.2C12 100ug
货号：
556433 基本售价：
5092.0 元 规格：
100ug
产品信息
概述| 货号 | 556433 |
| 描述 | A cytochrome is an electron-transporting protein that contains a heme prosthetic group. Cytochromes have been known to be essential components of the mitochondrial respiratory chain since 1925. The iron atom of the heme group in cytochromes alternates between a reduced ferrous (+2) state and an oxidized ferric (+3) state during electron transport in oxidative phosphorylation. Cytochromes are classified into four groups (a, b, c, and d) according to spectrochemical characteristics, and there are five cytochromes between Coenzyme QH2 and O2 in the electron transport chain. Cytochrome c is a water-soluble peripheral membrane protein of the mitochondria and transports electrons from the Coenzyme QH2 - Cytochrome c reductase complex to the Cytochrome c oxidase complex. Recently it has been suggested that Cytochrome c may be required for cells to undergo apoptosis. Mitochondria may be involved in apoptosis by releasing Cytochrome c to the cytosol where it functions in the activation of Caspase 3 (CPP32), an ICE family molecule that has been shown to trigger apoptosis when activated. In the cytoplasm Cytochrome C might exist in polymeric forms, appearing as a 58-60 kDa band by western blot analysis, rather than the monomeric form. Monomeric Cytochrome c migrates at a reduced molecular weight of 15 kDa. Clone 7H8.2C12 recognizes the denatured form of pigeon, horse, rat, mouse and human Cytochrome c. It does not recognize the native form of Cytochrome c. Clone 7H8.2C12 recognizes an epitope within amino acids 93-104 (inclusive) of pigeon Cytochrome C, based on competitive ELISA results. Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon Cytochrome c were used as immunogens. Clone 7H8.2C12 is used for western blot detection of Cytochrome c. Clone 6H2.B4, Cat. no. 556432, is used for immunoprecipitation. Cell lines P388D1 mouse lymphoma (ATCC CCL-46), HeLa human carcinoma (ATCC CCL-2), Jurkat T leukemia (ATCC TIB-152), and NIH/3T3 mouse fibroblast cells (ATCC CRL-1658) may be used as positive control cell lysates. |
| 同种亚型 | IgG2b |
| 反应种属 | Human (QC Testing) Mouse, Rat, Pigeon (Tested in Development) |
| 来源宿主 | Mouse |
| 使用方法 | WB |
性能| 供应商 | BD Pharmingen |
| 免疫原 | Synthetic Peptides of Pigeon Cytochrome C |
| 存放说明 | The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at 4°C.
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| 存储溶液 | Aqueous buffered solution containing ≤0.09% sodium azide. |
| 浓度 | 0.5 mg/ml |
