| 货号 | FAB42401V-100UG |
| 别名 | eGFP; Enhanced Green Fluorescent Protein; GFP; GFPuv; green fluorescent protein (gfp); Green Fluorescent Protein | 全称 | Green Fluorescent Protein |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects recombinant GFPuv and eGFP in Western blots. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant GFPuv Ser2-Lys238 Accession # P42212 |
| 标记 | Alexa Fluor 405 |
| 背景 | Green fluorescent protein (GFP) is a 27 kDa protein originally isolated from the jellyfish Aequoreavictoria. In the presence of UV light (490-520 nm), it emits a green fluorescent color that can be used to pinpoint locations of various intracellular proteins. GFP is 238 amino acids (aa) in length. It is a globular monomer that has a tendency to dimerize. The monomer has the shape of a beta -barrel with a chromophore (aa 65-67) containing alpha -helix running up its center. GFPuv is the Aequorea sequence with three aa substitutions; Phe to Ser at # 99, Met to Thr at # 153, and Val to Ala at # 163. This form expresses faster and is 18-fold brighter than native GFP; excitation peaks at 395 nm and emission at 508 nm. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
Detection of eGFP in HEK293 Human Cell Line Transfected with eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with eGFP were stained with and either (A) Mouse Anti-GFP Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB42401V) or (B) Mouse IgG1 Alexa Fluor 405 Isotype Control (Catalog # IC002V). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
