His Tag PerCP MAb (Clone AD1.1.10) (100 TESTS)
货号：
IC050C 基本售价：
5086.1 元 规格：
100 Tests
产品信息
概述| 货号 | IC050C |
| 别名 | polyHistidine |
全称 | Histidine Tag | | 应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells)
|
| 目标/特异性 | Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl‑terminus. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | His-tagged peptide |
| 标记 | PerCP |
| 背景 | Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Protein Purification Resin & Anti-poly Histidine Antibodies
|
参考图片 Detection of His Tag in NS0 Mouse Cell Line Transfected with His-tagged Protein by Flow Cytometry. NS0 mouse myeloma cell line transfected with His-tagged protein was stained with Mouse Anti-His Tag PerCP‑conjugated Monoclonal Antibody (Catalog # IC050C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
