| 货号 | IC42401A |
| 别名 | eGFP; Enhanced Green Fluorescent Protein; GFPuv; green fluorescent protein (gfp); Green Fluorescent Protein | 全称 | Green Fluorescent Protein |
| 应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects recombinant GFPuv and eGFP in Western blots. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant GFPuv Ser2-Lys238 Accession # P42212 |
| 标记 | Allophycocyanin |
| 背景 | Green fluorescent protein (GFP) is a 27 kDa protein originally isolated from the jellyfish Aequoreavictoria. In the presence of UV light (490-520 nm), it emits a green fluorescent color that can be used to pinpoint locations of various intracellular proteins. GFP is 238 amino acids (aa) in length. It is a globular monomer that has a tendency to dimerize. The monomer has the shape of a beta -barrel with a chromophore (aa 65-67) containing alpha -helix running up its center. GFPuv is the Aequorea sequence with three aa substitutions; Phe to Ser at # 99, Met to Thr at # 153, and Val to Ala at # 163. This form expresses faster and is 18-fold brighter than native GFP; excitation peaks at 395 nm and emission at 508 nm. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of GFPuv/eGFP in HEK293 Human Cell Line Transfected with eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with eGFP was stained with either (A) Mouse Anti-GFPuv/eGFP APC‑conjugated Monoclonal Antibody (Catalog # IC42401A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
