| 货号 | HCA210 |
| 克隆号 | AbD17827_hIgG1 |
| 同种亚型 | IgG1 |
| 来源宿主 | HuCAL |
| 应用 | E |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at -70oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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ELISA demonstrating the specificity of HCA210 using various antigens as coating reagents Antigens were coated at 5 µg/mL on a microtiter plate overnight. After washing and blocking with 5% milk powder in PBST, HRP-conjugated HCA210 (AbD17827_hIgG1; 20µL from a 2µg/mL solution in BUF049) was added. Detection was performed using the QuantaBlu™ fluorogenic peroxidase substrate | |
Inhibition of ustekinumab binding to IL-23 by HCA210 (clone AbD17827 in monovalent Fab-V5Sx2 format) IL-23 (1.0 µg/mL) was used as the coating antigen followed by a pre-incubated mixture of the anti-idiotypic antibody AbD17827 titrated into a constant amount of ustekinumab (0.3 µg/mL). Free ustekinumab still capable of binding to the plate was detected using Mouse anti Human IgG(Fc):HRP (MCA647P). Data is shown as the mean of three measurements | |
Human anti ustekinumab bridging ELISA for Anti-Drug Antibody (ADA) assay development A microtitre plate was coated over night with ustekinumab at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of Human anti ustekinumab (clone AbD17827_hIgG1). Detection was performed using HRP-conjugated ustekinumab at a concentration of 4 µg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. Data is shown as the mean of three measurements |
