| 货号 | MCA400 |
| 克隆号 | AA5H |
| 同种亚型 | IgG2a |
| 反应种属 | Viral |
| 来源宿主 | Mouse |
| 应用 | IF, P, WB |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Published customer image: Mouse anti Influenza A Nucleoprotein antibody, clone AA5H used for the detection of viral nucleoproitein in influenza infected HEK-293 cells by western blotting. Image caption: Forced expression of influenza viral proteins does not result in impaired IFNβ-induced STAT1 and STAT2 phosphorylation. HEK293 cells were transfected with 500 ng plasmid DNA for expression of viral NP, M, NS, (A) PA, PB1 and PB2 (C) genes (see Table 1 for accession numbers of viral genes) using L2000 according to manufacturer's instructions. Note that the Pol II constructs in use also give rise to expression of second reading frames in the NS, M and PB1 genes (NS2, M2, PB1-F2). 48 h post transfection cells were stimulated with human IFNβ (500 U/ml) for 15 minutes. Total protein lysates were subjected to Western blot analysis using anti-phospho-STAT1, anti-phospho-STAT2, anti-STAT1 antibodies. Expression of influenza viral proteins was monitored with antibodies against NP, M1, NS1, PA, PB1 or PB2. (E) HEK293 cells were infected with the human influenza virus PR8 (H1N1) (MOI = 5) for the indicated time points and were subsequently stimulated for 15 min with either human IFNβ at a concentration of 100 U/ml. Cell lysates were subjected to Western blots as described. (B, D, F) Quantification of relative pSTAT1 and pSTAT2 band intensities in A, C and E using AIDA software and 2D densitometry (Fuji). From: Pauli E-K, Schmolke M, Wolff T, Viemann D, Roth J, Bode JG, et al. (2008) Influenza A Virus Inhibits Type I IFN Signaling via NF-κB-Dependent Induction of SOCS-3 Expression. PLoS Pathog 4(11): e1000196. | |
Published customer image: Mouse anti Influenza A Nucleoprotein antibody, clone AA5H used for the detection of viral protein in a range of organs from an influenza infected patient using immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Image caption: The results of IHC stain with antibody against NP. Positive stain seen with diaminobenzidine (brown; Dako). Slides counterstained with haematoxylin. (A) Positive stain presented in pneumocytes of lung (arrow). (B) Endotheliocyte of aortopulmonary vessel (arrow). (C) Positive stain in nuclei and cytoplasm of axillary lymphoid-node (arrow). (D) Positive stain in lymphocytes of spleen (arrow). (E) Positive stain in cytoplasm of neuron and gliocyte from cerebral cortex (arrows). (F) Positive stain in nuclei and cytoplasm of intestine mucosa (arrows). (G) Positive stain in nuclei of mononuclear-like cells (arrows) with morphological features of macrophages from liver. (H) Positive stain in cytoplasm and nuclei of endotheliocytes of renal contex. (I) Positive stain in nuclei and cytoplasm of epithelial cells from ureter (arrow). Original magnifications: (A) ×20, (B–E) ×40, (F–G) ×20, (H–I) ×40. From: Gao R, Dong L, Dong J, Wen L, Zhang Y, Yu H, et al. (2010) A Systematic Molecular Pathology Study of a Laboratory Confirmed H5N1 Human Case. PLoS ONE 5(10): e13315. | |
Published customer image: Mouse anti Influenza A Nucleoprotein antibody, clone AA5H used for the identification of influenza viral nucleoprotein in infected cell lysates. Image caption: Replication and IFN-β induction by human and avian H5N1 strains in human cells. (A, B) A549 cells were infected at a multiplicity of 0.01 (A) or 1 (B) with the highly-pathogenic human H5N1 IAV isolates A/Hong Kong/156/1997 (HK/97) and A/Thailand/1 (KAN-1)/2004 (Thai/04), the avian H5N1 strains A/chicken/Indonesia/R132/2004 (Ch/Ind), A/duck/Vietnam/TG24-O1/2005 (Duck/VN) and A/common buzzard/Berlin/1/2006 (Buzz/Bln) as well as with the prototypic seasonal A/Panama/2007/99 (Pan/99) H3N2 virus and its mutant variant with a deleted NS1 gene (Pan-delNS1; shown only in panel A). Supernatant of infected cell cultures was sampled at the indicated time points and viral titers were determined by plaque assay. Mean data+SEM of at least three independent experiments is shown. (C) Immunoblot analysis of lysates from A549 cells infected at MOI = 1 using a viral NP-specific antibody at the indicated time points post infection (p.i.). Relative intensities of NP bands normalized to actin controls are depicted in comparison to Thai/04, which was arbitrarily set as 100%. (D, E) Concentrations of IFN-β in cell culture supernatants of A549 cells infected with the indicated viruses at low (MOI = 0,01, panel D) or high multiplicity (MOI = 1, panel E) determined via ELISA at 24 and 48 hpi (D) or at 16 hpi (E). Mean data of at least three independent experiments +/– SEM is shown. Symbols indicate significant differences between given results with p<0.05 (*), p<0.01 (**), or p<0.001(***), respectively, as determined by unpaired two-tailed t-tests. From: Matthaei M, Budt M, Wolff T (2013) Highly Pathogenic H5N1 Influenza A Virus Strains Provoke Heterogeneous IFN-α/β Responses That Distinctively Affect Viral Propagation in Human Cells. PLoS ONE 8(2): e56659. | |
Published customer image: Mouse anti Influenza A Nucleoprotein antibody, clone AA5H used for the identification of influenza viral nucleoprotein in infected cell lysates. Image caption: Published customer image: Mouse anti Influenza A Nucleoprotein antibody, clone AA5H used for the identification of influenza viral nucleoprotein in infected cell lysates. Image caption: H5N1 NS1 proteins inhibit the induction of type I IFN in human cells. (A) IFN-β concentrations of supernatants sampled from A549 cell cultures infected with the Pan99 WT and different NS reassortant viruses (H3N2) (MOI = 0.01) at 24 and 48 hrs p.i., (N=2+/–SEM). (B) Human 293T cells were co-transfected with plasmids expressing the H5N1- or Pan/99 (H3N2)-derived NS segments or empty vector, the human IFN-β promoter reporter plasmid p125-Luc and pRL-TK-Luc to control for transfection efficiency. Subsequently, cells were infected for 16 h with Pan-delNS1 to stimulate the IFN-β promoter before luciferase reporter activity was determined in cell lysates. IFN-β promoter activation in infected cells is shown as x-fold stimulation of firefly luciferase activity compared to transfected and mock infected cells. Mean data of three independent experiments is shown +/– SD. (C) Immunblot detection of viral NP after Pan-delNS1 (H3N2) infection confirmed equal stimulation of the transfected cells analyzed in panel B. From: Matthaei M, Budt M, Wolff T (2013) Highly Pathogenic H5N1 Influenza A Virus Strains Provoke Heterogeneous IFN-α/β Responses That Distinctively Affect Viral Propagation in Human Cells. PLoS ONE 8(2): e56659. |
