MOUSE ANTI HUMAN HLA ABC:Low Endotoxin【科瑞奥博】

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MOUSE ANTI HUMAN HLA ABC:Low Endotoxin

货号： MCA81EL 基本售价： 4885.0 元规格： 0.5 mg

产品信息

概述
货号	MCA81EL
克隆号	W6/32
同种亚型	IgG2a
反应种属	Human
来源宿主	Mouse
应用	C*, E, F, FN, IF, IP

性能
供应商	Bio-Rad Antibodies
溶解方法	Reconstitute with 1ml distilled water.
运输条件
存放说明	Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at -20^oC only This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

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参考图片

Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC (MCA81)

Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor^® 488 (MCA81A488)

Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor^® 647 (MCA81A647)

Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:FITC (MCA81F)

Staining of human peripheral blood monocytes with Mouse anti Human HLA ABC:Biotin (MCA81B)

Staining of peripheral blood lymphocytes with Mouse anti Human HLA-ABC: Low Endotoxin (MCA81EL)

Published customer image:
Mouse anti Human HLA ABC antibody, clone W6/32 used to immunoprecipitate all MHC-1 complexes from phagosomal complexes.
Image caption:
HLA-E:peptide complexes are present in phagosomal fractions. (A,B) DC were pulsed with H37Rv-eGFP or CFP102–11 peptide and the homogenate was separated using 27% percoll as described. Each fraction was freeze-thawed and tested for its ability to stimulate D160 1-23 (A) or D160 1-1B (A&B) CD8+ T cell clones in the absence of additional APC. IFN-? production was measured using ELISPOT. The mean±SEM of duplicate wells is presented. Data are representative of four experiments at various timepoints in A, and four experiments after a one hour peptide pulse in B. (C) Plasma membrane/ER fractions from Mtb–infected DC (HLA-B15+/HLA-B44-) were incubated with HLA-I matched or mismatched CD8+ T cell clones and IFN-? production was measured using ELISPOT. The mean±SEM of duplicate wells is presented and data are representative of three experiments done similarly. (D,E) Individual fractions were analyzed by flow cytometry to assess HLA-II-PE (plasma membrane) and H37Rv-eGFP fluorescence. Selected fractions are shown (D) including the peak plasma membrane fraction (#8) and phagosomal fractions (#24–27). Prior to flow cytometry, fractions were mixed with a reference latex bead population at a known concentration. Equal numbers of latex bead events were collected for all fractions and used to quantify the number of plasma membrane and phagosome particles (E) as described in Materials and Methods. (F) HLA-I was immunoprecipitated from plasma membrane or phagosome fractions using W6/32. After dilution of the plasma membrane sample to give similar levels of HC10 staining, the presence of HLA-I alleles was assessed using antibodies to pan-HLA-I (HC10), HLA-A2 (HCA2), and HLA-E (MEM-E/02). The numbers below blots indicate the relative intensity of the bands as described in Materials and Methods.
From: Grotzke JE, Harriff MJ, Siler AC, Nolt D, Delepine J, et al. (2009) The Mycobacterium tuberculosis Phagosome Is a HLA-I Processing Competent Organelle.
PLoS Pathog 5(4): e1000374.