Digoxigenin Alexa Fluor 488 MAb (Clone 611621) (100 TESTS)
货号：
IC7520G 基本售价：
4808.5 元 规格：
100 Tests
产品信息
概述| 货号 | IC7520G |
| 别名 | DIG |
| 应用 | Immunohistochemistry(1:10 dilution)
|
| 目标/特异性 | Detects Digoxigenin labeled proteins, nucleic acids and Digoxigenin conjugated primary and secondary antibodies. |
| 使用方法 | Immunohistochemistry: 1:10 dilution
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | KLH-coupled Digoxigenin |
| 标记 | Alexa Fluor 488 |
| 背景 | Digoxigenin is a hapten, a small molecule with high immunogenicity, that is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein. Digoxigenin is a steroid found exclusively in the flowers and leaves of the plant genus Digitalis. Digoxigenin can be introduced into proteins and nucleic acids for detection in a variety of assays, including ELISA, Immunohistochemistry, in situ hybridization, Southern blot, and Western blot. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Décarie, A.A. et al. (1994) Peptides 15(3):511.
- Hauptmann, G. et al. (1994) Trends in Genetics 10(8):266.
- Goodarzi, M.T. et al. (1995) Biochemical Society Transactions 23(2):168S.
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参考图片Immunohistochemistry Netrin-4 was detected in perfusion fixed frozen sections of mouse brain (nucleus accumbens) using digoxigenin-conjugated Goat Anti-Mouse Netrin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1132) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Digoxigenin Alexa Fluor® 488-conjugated Monoclonal Antibody (green; Catalog # IC7520G) at a final concentration of 1X (1:10 dilution) and counterstained with DAPI (blue). Specific staining was localized to vasculature. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
