MOUSE ANTI PIG wCD8 ALPHA:FITC【科瑞奥博】

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MOUSE ANTI PIG wCD8 ALPHA:FITC

货号： MCA1223F 基本售价： 4568.0 元规格： 0.1 mg

产品信息

概述
货号	MCA1223F
克隆号	MIL12
同种亚型	IgG2a
反应种属	Pig
来源宿主	Mouse
应用	F

性能
供应商	Bio-Rad Antibodies
溶解方法	Reconstitute with 1.0 ml distilled water
运输条件
存放说明	Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

声明
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参考图片

Pig peripheral blood lymphocytes stained with Mouse anti Pig wCD8a (MCA1223GA) followed by Goat anti Mouse IgG (H/L):FITC (STAR117F)

Published customer image:
Mouse anti Pig wCD8 alpha antibody, clone MIL12 (MCA1223) used for the evaluation of CD7α expression on porcine lymphocytes by flow cytometry.
Image caption:
Frequency of leukocyte subsets in PBMC and LPMC using FCM analysis A) shows a representative FCM analysis (n=4) from PBMCs (left) and LPMC (right) isolated from 6 week old pigs were analysed on the frequencies of different immune cell subsets using three-color FCM. Forward scatter (FSC) and side scatter (SSC) were used to gate on leukocytes (PBMCs (a) and LPMC (h) for further analysis of myeloid cells (CD172+; b and i), NK cells (CD3- CD8α+; c and j), B cells (CD21+; e and l) and T cells (CD3+; d and k). Gated T cells were then further analysed on their CD4/CD8α expression to determine the frequency of CTLs (CD4-CD8αhigh; f and m) and CD4+ T cells (f and m). CD8α expression of CD4+ T cells was also investigated to define the frequency of experienced CD4+ T cells (CD8a+CD4+; g and n). (o) Summarizes the results of this FCM analysis of all four analysed animals. Percentages from the PBMC population are represented by a closed circle and the percentages from the LPMC populations are represented by an open box. (p) and (q) is a pie chart showing the relative average percentage of PBMCs and LPMCs from all 4 animals.

From: Pasternak JA, Ng S, Kaser T, Meurens F, Wilson HL (2014) Grouping Pig-Specific Responses to Mitogen with Similar Responder Animals may Facilitate the Interpretation of Results Obtained in an Out-Bred Animal Model.
J Vaccines Vaccin 5:242.

Figure A. FITC conjugated mouse anti porcine CD5 (MCA2307F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti porcine CD5 (MCA2307F) and RPE conjugated mouse anti porcine CD8a (MCA1223PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Figure A. RPE conjugated mouse anti porcine CD8a (MCA1223PE) and FITC conjugated mouse IgG2a isotype control (MCA929F). Figure B. RPE conjugated mouse anti porcine CD8a (MCA1223PE) and FITC conjugated mouse anti porcine CD5 (MCA2307F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.